Ripm 1640

Irradiated and control mice were sacrificed by pentobarbital injection. BALF from mice was collected as described previously and centrifuged at 500x g for 5 min at 4°C, supernatants were stored at –80°C for ELISA (Zhang et al., 2016b (link)).
AMs were isolated as described previously (Guo et al., 2017 (link)). Briefly, cell pellets from BALF were re-suspended in RIPM 1640 (Gibco) with 10% heat-inactivated fetal bovine serum (FBS, Biowest) and 50 units/ml penicillin and streptomycin (Gibco), then plated for 2 h; non-adherent cells were washed out with PBS, and macrophages were enriched ∼90%.

The human GC cell lines, AGS, SNU5 and SNU16, were purchased from the Cell Bank of Chinese Academy of Science and Bena Culture Collection. AGS is an adherent cell line, while SNU5 and SNU16 are semi-adherent and suspended cells respectively. AGS cells were cultivated in high-glucose DMEM (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 U/ml penicillin and 100 ng/ml streptomycin. SNU5 and SNU16 cells were maintained in RIPM1640 (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS. All cell lines were subjected to STR analysis and the absence of mycoplasma contamination was confirmed. The three cell lines were cultivated at 37°C with 5% CO₂. Organoids derived from MA were constructed as previously described (27 (link)).