Digidata 1322 a ad da converter

Microelectrodes made from capillary pipettes were cut with resistance in the 0.3–2 MΩ range and filled with 3 M KCl. An oocyte was penetrated by two electrodes and whole-oocyte currents were measured with perfusion of extracellular solutions. Currents and voltages were obtained using a Geneclamp 500B amplifier and Digidata 1322 A AD/DA converter (Molecular Devices, Union City, CA) at 200 μs/sample and Bessel filtered at 2 kHz. The files were analyzed with pClamp 11 (Axon Instruments, Union City, CA) and data plotted with GraphPad Prism 8 (GraphPad Software, San Diego, CA).

Two-microelectrode voltage clamp experiments were performed as described before9 (link). Briefly, the two electrodes (Capillary pipettes, Warner Instruments, Hamden, CT) impaling oocytes were filled with 3 M KCl to form a tip resistance of 0.3–2 MΩ. The standard extracellular solution (pH 7.5) containing 100 mM NaCl, 2 mM KCl, 1 mM MgCl₂ and 10 mM HEPES was used. The solution containing extracellular Ca²⁺ was prepared from the standard solution with the addition of CaCl₂ to a final concentration of 5 mM. Duration of application of Ca²⁺ medium was indicated in time course recordings. Oocyte whole-cell currents were recorded using a Geneclamp 500B amplifier and Digidata 1322A AD/DA converter (Molecular Devices, Union City, CA). The pClamp 9 software (Axon Instruments, Union City, CA) was applied for data acquisition and analysis. Currents and voltages were digitally recorded at 200 μs/sample and filtered at 2 kHz through a Bessel filter. SigmaPlot 12 (Systat Software, San Jose, CA) was used for data fitting and plotting.

Two-electrode voltage clamp experiments using oocytes were carried out as previous^{61 (link)}. Briefly, two electrodes made of capillary pipettes (Warner Instruments, Hamden, CT) penetrating an oocyte were filled with 3 M KCl to obtain a tip resistance ranging from 0.3 to 2 MΩ. Whole-cell currents of an oocyte were recorded at RT in an extracellular solution with composition specified in figure legends. Electric currents were recorded with a Geneclamp 500B amplifier and Digidata 1322 A AD/DA converter (Molecular Devices, Union City, CA). Currents and voltage were recorded at 200 μs/sample and Bessel filtered at 2 kHz. The data was acquainted and analyzed with software pClamp version 9 (Axon Instruments, Union City, CA). SigmaPlot version 13 (Systat Software, San Jose, CA) and GraphPad Prism version 7 (GraphPad Software, San Diego, CA) were employed for data plotting.