Western blots were performed using standard methods. Blots were incubated with primary antibodies against: anti-GFP (Santa Cruz Biotechnology, 1:500), anti-MUS81 (Santa Cruz Biotechnology, 1:1000), anti-LAMIN B1 (Abcam, 1:10,000), anti-GAPDH (Millipore, 1:5000), anti-RAD51 (Abcam 1:10,000), anti-SMARCAL1 (Bethyl 1:1500), anti-ZRANB3 (Proteintech 1:1000), anti-BRCA2 (Bethyl 1:5000), anti-POLA1 (Polα - Bethyl, 1:500), anti-WDHD1 (Novus Biologicals, 1:1000). After incubations with horseradish peroxidase-linked secondary antibodies (Jackson ImmunoResearch, 1:20,000), the blots were developed using the chemiluminescence detection kit ECL-Plus (Amersham) according to the manufacturer’s instructions. Quantification was performed on blot acquired by ChemiDoc XRS+ (Bio-Rad) using Image Lab software, the normalization of the protein content was done through LAMIN B1 or GAPDH immunoblotting.
Anti zranb3
Manufactured by Proteintech
Anti-ZRANB3 is a primary antibody that detects the ZRANB3 protein. ZRANB3 is a DNA-dependent ATPase enzyme involved in DNA damage response and replication fork stability. The Anti-ZRANB3 antibody can be used to study the expression and localization of ZRANB3 in various cell and tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
Chemidoc xr, by Bio-Rad (2 mentions)
Ecl plu, by Cytiva (2 mentions)
Horseradish peroxidase linked secondary antibody, by Jackson ImmunoResearch (2 mentions)
Anti gapdh, by Merck Group (2 mentions)
Anti smarcal1, by Fortis Life Sciences (2 mentions)
Lamin b1, by Bio-Rad (2 mentions)
Gapdh, by Bio-Rad (2 mentions)
Anti mus81, by Santa Cruz Biotechnology (2 mentions)
Anti rad51, by Abcam (1 mentions)
Anti lamin b1, by Abcam (1 mentions)
Anti brca2, by Fortis Life Sciences (1 mentions)
Anti gfp, by Santa Cruz Biotechnology (1 mentions)
Lamin b1, by Abcam (1 mentions)
Anti rpa32, by Santa Cruz Biotechnology (1 mentions)
Anti rad51, by Bioss Antibodies (1 mentions)
Anti rad52, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti zranb3
1
Western Blotting Protein Detection
2
Western Blot Analysis of DNA Repair Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Western blots were performed using standard methods. Blots were incubated with primary antibodies against: anti-MUS81 (Santa Cruz Biotechnology, 1:2000), Lamin B1 (Abcam, 1:10,000), anti-RAD52 (Santa Cruz Biotechnology, 1:500), anti-GAPDH (Millipore, 1:5000), anti-RAD51 (Bioss 1:1000), anti-SMARCAL1 (Bethyl 1:1000), anti-RPA32 (Santa Cruz Biotechnology 1:1000) and anti-ZRANB3 (Proteintech 1:1000). After incubations with horseradish peroxidase-linked secondary antibodies (Jackson ImmunoResearch, 1:30,000), the blots were developed using the chemiluminescence detection kit ECL-Plus (Amersham) according to the manufacturer’s instructions. Quantification was performed on blot acquired by ChemiDoc XRS+ (Bio-Rad) using Image Lab software, and values shown on the graphs represent a normalisation of the protein content evaluated through Lamin B1 or GAPDH immunoblotting. A complete list of antibodies and dilutions is provided in Supplementary Table 2 and as Souce data file.
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