The miR-21‑5p mimic, si-PDCD4 and the corresponding NC sequences were purchased from GenePharma (Guangzhou, China). Briefly, CC cells were seeded in a six-well plate. When the cells were 70–80% confluent, the miR-21 inhibitor, miR-21 inhibitor NC, miR-21 mimic, miR-21 mimic NC, or si-PDCD4 at a final concentration of 20 nM was transfected into HeLa or SiHa cells using Lipofectamine™ 3000 (Invitrogen/Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s instructions. For the rescue experiment, the miR-21 mimic or NC was transfected into cell lines stably transfected with GAS5. Functional experiments were performed after transfection for 48 h.
Si pdcd4
Manufactured by Thermo Fisher Scientific
Sourced in United States
Si-PDCD4 is a small interfering RNA (siRNA) targeting the PDCD4 gene. It is designed to enable the knockdown of PDCD4 expression in cell-based studies.
Automatically generated - may contain errors
Lab products found in correlation
Si pdcd4, by GenePharma (2 mentions)
Mir 21 mimic, by Thermo Fisher Scientific (1 mentions)
Mir 21 5p mimic, by GenePharma (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Control mimic, by Thermo Fisher Scientific (1 mentions)
Sicontrol, by Thermo Fisher Scientific (1 mentions)
Pcdna3.1 empty vector, by GenePharma (1 mentions)
Si control, by GenePharma (1 mentions)
Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions)
Mir mimic control, by GenePharma (1 mentions)
Mir 499a 5p inhibitor, by GenePharma (1 mentions)
Mir inhibitor control, by GenePharma (1 mentions)
Mir 499a 5p mimic, by GenePharma (1 mentions)
2 protocols using si pdcd4
1
MiR-21 Mimic and PDCD4 Silencing
2
Regulation of LINC01018 and miR-499a-5p in HL-60 and THP-1 cells
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pcDNA3.1/LINC01018, a miR-499a-5p mimic (5′-UUAAGACUUGCAGUGAUGUUU-3′), miR-499a-5p inhibitor (5′-AAACATCTCTGCAAGTCTTAA-3′), miR mimic control (5′-ACUACUGAGUGACAGUAGA-3′), miR inhibitor control (5′-CAGUACUUUUGUGUAGUACAA-3′), si-PDCD4 (sense, 5′-GGAGCGGUUUGUAGAAGAAdTdT-3′ and antisense, 3′-dTdTCCUCGCCAAACAUCUUCUU-5′), si-control (sense, 5′-UUCCCUUUGUCAUCCUUUGCCUdTdT-3′ and antisense, 3′-dTdTAAGGGAAACAGUAGGAAACGGA-5′) and a pcDNA3.1 empty vector were all purchased from Shanghai GenePharma Co., Ltd. HL-60 and THP-1 cells were transfected with pcDNA3.1 (50 ng), pcDNA3.1/LINC01018 (50 ng), miR-499a-5p mimic (100 nM), mimic control (100 nM), miR-499a-5p inhibitor (100 nM), inhibitor control (100 nM), si-PDCD4 (50 nM), and si-control (50 nM) at 37°C for 48 h using Lipofectamine 3000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.). Cells were collected and used 48 h later for subsequent experimentation.
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