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Open array plate

Manufactured by Thermo Fisher Scientific
Sourced in United States

The Open-Array plate is a laboratory equipment designed for high-throughput molecular analysis. It enables the simultaneous analysis of multiple samples and targets, facilitating efficient and accurate data generation. The core function of the Open-Array plate is to provide a platform for conducting various types of molecular assays, such as gene expression, genotyping, and SNP analysis. The plate is designed to optimize workflow and maximize the number of data points obtained per experiment.

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2 protocols using open array plate

1

Multiplex qPCR Analysis of Intestinal Health

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A custom Open-Array plate (Applied Biosystems, Foster City, CA, USA) was designed with a total of 56 selected genes related to intestinal health (Supplementary Table S4). Details regarding genes and primers can be found in previous published work55 . Multiplex real-time qPCRs were performed in a QuantStudio 12 K Flex Real-Time PCR system (ThermoFisher Scientific, Waltham, MA) using TaqMan Open-Array Real-Time PCR Custom Assays. A final cDNA volume of 6 μl from each sample was transferred to 384-well plates and analysed per duplicate. One sample was used as an inter-plate control to check the replication of results from different plates.
Gene expression data analysis was performed as specified by Reyes-Camacho et al55 .
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2

Gene Expression Analysis of Intestinal Health

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The expression of a total of 52 genes related to intestinal health was studied through a custom OpenArray plate (Applied Biosystems, Foster City, CA, USA). Full details of the method has been previously described by González-Solé et al81 (link). The method had been optimized to quantify the expression of relevant genes in jejunum. Genes were selected based on previous knowledge and other reported works in the literature. GAPDH, ACTB, TBP and B2M were used as housekeeping genes. RNA was extracted from 100 mg of frozen jejunum tissue with the RiboPure kit (Ambion, Foster City, CA, USA) following the manufacturer’s protocol. A final cDNA volume of 6 μl from each sample was transferred per duplicate to a TaqMan OpenArray custom plate for gene expression and run in a QuantStudio 12 K Flex Real-Time PCR System (ThermoFisher Scientific, Waltham, MA, USA). Gene expression data analysis was performed as specified by González-Solé et al81 (link). A list of the 52 genes included in the custom plate can be found in Supplementary information (Supplementary Table S1).
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