Anti erα

Manufactured by Agilent Technologies

Sourced in United States

Anti-ERα is a laboratory equipment product designed for the detection and quantification of the estrogen receptor alpha (ERα) protein. This product enables researchers to study the expression and regulation of ERα, which is a key regulator of estrogen signaling and plays a crucial role in various biological processes and diseases.

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Lab products found in correlation

Vector abc system, by Vector Laboratories (2 mentions) Anti sma, by Merck Group (1 mentions) Anti fscn1, by Santa Cruz Biotechnology (1 mentions) Anti hmb 45, by Santa Cruz Biotechnology (1 mentions) Anti ps6 clone 91b2, by Cell Signaling Technology (1 mentions) Anti id 1, by Santa Cruz Biotechnology (1 mentions) Anti pr, by Agilent Technologies (1 mentions) Anti sox9, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-TM (1 citations)

4 protocols using anti erα

Immunohistochemical Analysis of Molecular Markers

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The antibodies used in this study were anti-ERα (#IR151, Dako), anti-FSCN1 (#SC-56531, Santa Cruz Biotechnology), anti-HMB-45 (#SC-59305, Santa Cruz Biotechnology), anti ID1 (#SC-488, Santa Cruz Biotechnology), anti-PR (#IR168, Dako), anti-phospho-Ser235-236 S6 ribosomal protein (anti-pS6; clone 91B2, Cell Signaling Technology), anti-SMA (#A2547, Sigma-Aldrich), and anti-SOX9 (#AB5535, Millipore).

Nuñez O., Román A., Johnson S.R., Inoue Y., Hirose M., Casanova Á., de Garibay G.R., Herranz C., Bueno-Moreno G., Boni J., Mateo F., Petit A., Climent F., Soler T., Vidal A., Sánchez-Mut J.V., Esteller M., López J.I., García N., Gumà A., Ortega R., Plà M.J., Campos M., Ansótegui E., Molina-Molina M., Valenzuela C., Ussetti P., Laporta R., Ancochea J., Xaubet A., Pollán M, & Pujana M.A. (2016). Study of breast cancer incidence in patients of lymphangioleiomyomatosis. Breast Cancer Research and Treatment, 156, 195-201.

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Immunohistochemical Analysis of Mouse Tissues

Cited 3 times

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Mouse tissues were dissected and fixed in 4% PFA/PBS for 2 days at 4°C, dehydrated through ethanol, and embedded in paraffin. Eight-μm serial sections were then prepared for histological analysis. Immunohistochemical analyses were carried out by standard procedures using the following antibodies (Ab) [14 (link)]: anti-beta-catenin (1:1000, cat. no. C19220, BD Biosciences) [43 (link)], anti-Lef1 (1:200, cat. no. 2230, CST) [43 (link)], anti-SMA (1:100, cat. no. M0851, Dako) [32 (link)], and anti-ERα (1:100, cat. no. 1115-1, Epitomics) [36 (link)].

Haraguchi R., Kitazawa R., Murashima A., Yamada G, & Kitazawa S. (2017). Developmental Contribution of Wnt-signal-responsive Cells to Mouse Reproductive Tract Formation. Acta Histochemica et Cytochemica, 50(4), 127-133.

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Immunohistochemical Analysis of Penile Estrogen Receptor Alpha

Cited 1 time

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Immunohistochemistry was carried out on penises of 10-day untreated mice. Penile specimens were formalin fixed, paraffin embedded and serially sectioned at 7μm. Immunohistochemistry was carried out as previously described (Rodriguez et al., 2012 (link)) utilizing anti-ERα (Dako, Carpinteria, CA, USA). Signal detection was achieved using the Vector ABC System (Vector Laboratories, Foster City, CA, USA) followed by exposure to diaminobenzidine (Sigma®). Sections exposed to all steps except the application of the primary antibodies were used as negative controls.

Mahawong P., Sinclair A., Li Y., Schlomer B., Rodriguez E J.r., Ferretti M.M., Liu B., Baskin L.S, & Cunha G.R. (2014). Comparative effects of neonatal diethylstilbestrol on external genitalia development in adult males of two mouse strains with differential estrogen sensitivity. Differentiation; research in biological diversity, 88(0), 70-83.

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Androgen Receptor Immunohistochemistry Protocol

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Methods for androgen receptor immunohistochemistry (IHC) have been described (Cunha et al., 2005 (link)). Tissue sections were de-paraffinized in Histoclear (National Diagnostic, Atlanta, GA) and hydrated in graded alcohols and phosphate-buffered saline (PBS). Endogenous peroxidase activity was blocked with 0.5% hydrogen peroxide in methanol for 30 minutes. Normal goat serum was applied to the sections for 30 minutes to bind nonspecific sites. The sections were then incubated with the primary antibodies overnight at 4°C or with non-immune serum or mouse IgG. The following antibodies were used: a rabbit polyclonal anti-androgen receptor antibody (Affinity BioReagents, Golden, CO); anti-ERα (mouse monoclonal clone 1D5, diluted 1:30, Dako, Carpinteria, CA, USA); and anti-ERβ (mouse monoclonal clone EMR02, diluted 1:200, Leica Microsystems, Newcastle Upon Tyne, UK). Signal detection was achieved using the Vector ABC System (Vector Laboratories, Foster City, CA, USA) followed by exposure to diaminobenzidine (Sigma, St. Louis, MO). Sections exposed to all steps except the application of the primary antibodies were used as negative controls.

Cunha G.R., Risbridger G., Wang H., Place N.J., Grumbach M., Cunha T.J., Weldele M., Conley A.J., Barcellos D., Agarwal S., Bhargava A., Drea C., Hammond G.L., Siiteri P., Coscia E.M., McPhaul M.J., Baskin L.S, & Glickman S.E. (2014). Development of the External Genitalia: Perspectives from the Spotted Hyena (Crocuta crocuta). Differentiation; research in biological diversity, 87(0), 4-22.

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