Microrna mimics

Manufactured by Merck Group

Sourced in Sao Tome and Principe

MicroRNA mimics are synthetic molecules designed to mimic the structure and function of naturally occurring microRNAs. They are used in research and development applications to study gene expression and regulation.

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Lab products found in correlation

Hrp conjugated secondary antibody, by Jackson ImmunoResearch (2 mentions) Enhanced chemi luminescence (ecl), by Cytiva (2 mentions) Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (2 mentions) Caco 2, by American Type Culture Collection (1 mentions) Taqman probe, by Thermo Fisher Scientific (1 mentions) Ncm460, by American Type Culture Collection (1 mentions) 14c aa, by PerkinElmer (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Anti actin antibody, by Santa Cruz Biotechnology (1 mentions) Bodipy 493 503, by Thermo Fisher Scientific (1 mentions) Lipidtox, by Thermo Fisher Scientific (1 mentions) Psicheck2 reporter plasmid, by Promega (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

MISSION® microRNA Mimic (14 citations) MISSION® microRNA Mimic hsa-miR-21 (2 citations) MISSION® microRNA Mimic hsa-miR-152 (2 citations)

4 protocols using microrna mimics

Radioactive Amino Acid Uptake Assay

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¹⁴C-AA (specific activity 2.8 mCi/mmol; radiochemical purity > 97%) was from Perkin Elmer (Boston MA). Caco-2 and NCM460 cells were from American Type Culture Collection (ATCC, Manassas, VA) and INCELL (San Antonio, TX), respectively. Primers for PCR amplifications, microRNA mimics and inhibitors were from Sigma (Sigma, St. Louis, MO/Thermo Fisher, Huntington Beach, CA). TaqMan probes and primers for mature microRNAs amplification were from Applied Biosystems (Foster City, CA). The monoclonal hSVCT1 antibody was from Santa Cruz Biotechnology Inc., (Santa Cruz, CA).

Subramanian V.S., Sabui S., Marchant J.S, & Said H.M. (2018). MicroRNA-103a regulates sodium-dependent vitamin C transporter-1 expression in intestinal epithelial cells. The Journal of nutritional biochemistry, 65, 46-53.

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Lipid Droplet Regulation via Rab GTPases

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Unless otherwise stated, all reagents were obtained from Sigma Chemical Co. (St.Louis, MO). Tissue culture supplies were obtained from the Grand Island Biological Co. (Grand Island, NY). Lipofectamine 3000 and Lipofectamine RNAi max reagent were purchased from Thermo Fisher Scientific. pmirGLO Dual-Luciferase miRNA Target Expression Vector and Dual-Luciferase Reporter Assay System were purchased from Promega Life Science (Madison, WI). Anti-Rab18 antibody and microRNA mimics were obtained from Sigma Aldrich (St. Louis, MO). Antibodies against Perilipin, Rab5a, Rab27 were purchased from Abcam (Cambridge, England). Anti-actin antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies against Lamp1, Rab7, Rab9 and Dicer were obtained from Cell Signalling Technologies (Danver, MA). Anti-Rab4 and anti-Rab8 antibodies were acquired from BD Biosciences, USA. All HRP-conjugated secondary antibodies were purchased from Jackson Immuno-Research Laboratory (West Grove, PA) and ECL was obtained from Amersham Biosciences, UK. Alexa labelled secondary antibodies for immunofluorescence studies, BODIPY 493/503 and LipidTOX were purchased from Molecular Probes (Eugene, OR). Linolenic acid-oleic acid-albumin (100x) was obtained from Sigma Aldrich (St. Louis, MO). All other reagents used were of analytical grade.

Sood C., Verma J.K., Basak R., Kapoor A., Gupta S, & Mukhopadhyay A. (2024). Leishmania highjack host lipid body for its proliferation in macrophages by overexpressing host Rab18 and TRAPPC9 by downregulating miR-1914-3p expression. PLOS Pathogens, 20(2), e1012024.

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Validating miR-9 Binding to FOXP1 3' UTR

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The FOXP1 3’ UTR, a miR-9 full length binding site (23 nt) and a mutant miR-9 binding site with 4 mismatched nucleotides were cloned between Xho1 and Not1 restrictions sites of the psiCHECK™-2 reporter plasmid (Promega, Madison, WI). Between 5 × 10⁴ and 1 × 10⁵ cells were plated into 24 well plates. Cells were co-transfected with 100nm reporter plasmid and 100nM microRNA mimics (Sigma-Aldrich, St. Louis, MO) or 100nM miR-9 Locked Nucleic Acids (Exiqon Inc., Woburn, MA). Relative luciferase activity was determined 30hrs after transfection.

Gomez G.G., Volinia S., Croce C.M., Zanca C., Li M., Emnett R., Gutmann D.H., Brennan C.W., Furnari F.B, & Cavenee W.K. (2014). Suppression of MicroRNA-9 by Mutant EGFR Signaling Upregulates FOXP1 to Enhance Glioblastoma Tumorigenicity. Cancer research, 74(5), 1429-1439.

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Regulation of Rab GTPases in Endocytic Pathways

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Unless otherwise stated, all reagents were obtained from Sigma Chemical Co. (St. Louis, MO). Tissue culture supplies were obtained from the Grand Island Biological Co. (Grand Island, NY). Lipofectamine 2000 and Lipofectamine RNAi max reagent were purchased from Thermo Fisher Scientific. pmirGLO Dual-Luciferase miRNA Target Expression Vector and Dual-Luciferase Reporter Assay System were purchased from Promega Life Science (Madison, WI). microRNA mimics were obtained from Sigma Aldrich (St. Louis, MO). Antibodies against Rab5a and Rab5c were purchased from Abcam (Cambridge, England) whereas anti-Rab5b antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies against Rab5, Rab7, Rab9, c-Jun, Lamp1 were obtained from Cell Signaling Technologies (Danver, MA). Anti-Rab8 and anti-Rab11 antibodies were purchased from BD Biosciences, USA. Anti-EEA1 antibody was received as kind gift from Dr. Marino Zerial (Max Planck Institute, Dresden, Germany). All HRP-conjugated secondary antibodies were purchased from Jackson ImmunoResearch Laboratory (West Grove, PA) and ECL was obtained from Amersham Biosciences, UK. All secondary antibodies used for immunofluorescence studies were purchased from Molecular Probes (Eugene, OR). All other reagents used were of analytical grade.

Verma J.K., Rastogi R, & Mukhopadhyay A. (2017). Leishmania donovani resides in modified early endosomes by upregulating Rab5a expression via the downregulation of miR-494. PLoS Pathogens, 13(6), e1006459.

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