At the CML, all samples were re-analyzed by CMIA for HBsAg using ARCHITECT HBsAg (Abbott Laboratories, IL,USA) on ARCHITECT i4000SR platform according to the instructions of the manufacturer.
The samples were also analyzed with CMIA for anti-HBs and total anti-HBc using ARCHITECT anti-HBs and ARCHITECT anti-HBc II (Abbott Laboratories, IL, USA), respectively on the ARTCHITECT i4000SR machine following the manufacturer’s instructions. HBsAg positive samples were further analyzed for HBeAg and anti-HBe with the ARCHITECT HBeAg and ARCHITECT anti-HBe (Abbott Laboratories, IL, USA) assays. All HBsAg positive and isolated anti-HBc-positive samples were analyzed for antibodies against hepatitis delta virus (anti-HDV) with the ETI-AB-DELTA K-2 kits (Diasorin, Saluggia, Italy) according to the instructions by the manufacturer.
The samples were also analyzed with CMIA for anti-HBs and total anti-HBc using ARCHITECT anti-HBs and ARCHITECT anti-HBc II (Abbott Laboratories, IL, USA), respectively on the ARTCHITECT i4000SR machine following the manufacturer’s instructions. HBsAg positive samples were further analyzed for HBeAg and anti-HBe with the ARCHITECT HBeAg and ARCHITECT anti-HBe (Abbott Laboratories, IL, USA) assays. All HBsAg positive and isolated anti-HBc-positive samples were analyzed for antibodies against hepatitis delta virus (anti-HDV) with the ETI-AB-DELTA K-2 kits (Diasorin, Saluggia, Italy) according to the instructions by the manufacturer.