Thca a

Stocks of 1 mg/mL of CBD, THC, CBN, 11-OH-THC, CBG, THCV, THCA-A, and THCCOOH-D 3 as well as 100 μg/mL solutions of THCCOOH, THCCOOH-gluc, CBD-D 3 , THC-D 3 , CBN-D 3 , and 11-OH-THC-D 3 were purchased from Cerilliant (Round Rock, TX, USA). A 10-μg/mL THCgluc stock was obtained from ElSohly (Oxford, MS, USA).
Optima LC/MS grade acetonitrile, methanol, 2-propanol, formic acid (FA), and ammonium acetate were obtained from Fisher Scientific (Hampton, NH, USA). Oasis PRiME HLB 96-well solid-phase extraction (SPE) plates with 30 mg sorbent/well, 96-well collection plates, quick-load glass inserts, silicone/PTFE-treated 96-well square plug cap mats, and amber max recovery autosampler vials were acquired from Waters (Milford, MA, USA). Borosilicate glass tubes were from Fisher Scientific and 18.2 MΩ-cm water was dispensed from a Siemens PureLab Ultra purification system.
Blank WB was created by mixing acid citrate dextrose (ACD)packed red blood cells with ABO-compatible plasma and confirming the absence of detectable cannabinoids. Breath matrix consisted of an unused SensAbues ® electret filter material ("pad") cut into 28 cm 2 squares to match the intake surface area of the SensAbues ® collection device.