Cells harvested from colony forming assay were washed in PBS and lysed in TRI Reagent (Zymo Research). RNA extraction was performed using Direct-zol RNA Kits (Zymo Research) according to the protocol of the manufacturer. RNA concentration and quality were determined with the Agilent 2100 Bioanalyzer system using the Eukaryote Total RNA Pico Assay kit. RNA sequencing libraries were prepared using Takara SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian kit according to the manufacturer’s instructions. The quality and concentration of final libraries were assessed using Agilent 2100 Bioanalyzer system and Qubit DNA HS assay, respectively.
Qubit dna hs assay
Manufactured by Agilent Technologies
The Qubit DNA HS assay is a fluorescence-based quantitation kit designed to measure the concentration of DNA samples. It provides a sensitive and accurate method for quantifying DNA in the range of 0.2 to 100 ng.
Automatically generated - may contain errors
Lab products found in correlation
Total rna pico assay kit, by Agilent Technologies (2 mentions)
Smarter stranded total rna seq kit v2 pico input mammalian kit, by Takara Bio (2 mentions)
Direct zol rna kit, by Zymo Research (2 mentions)
Agilent bioanalyzer 2100 system, by Agilent Technologies (2 mentions)
Tri reagent, by Zymo Research (2 mentions)
2 protocols using qubit dna hs assay
1
RNA Extraction and Sequencing Library Prep
2
RNA Extraction and Sequencing Library Prep
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Cells harvested from colony forming assay were washed in PBS and lysed in TRI Reagent (Zymo Research). RNA extraction was performed using Direct-zol RNA Kits (Zymo Research) according to the protocol of the manufacturer. RNA concentration and quality were determined with the Agilent 2100 Bioanalyzer system using the Eukaryote Total RNA Pico Assay kit. RNA sequencing libraries were prepared using Takara SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian kit according to the manufacturer’s instructions. The quality and concentration of final libraries were assessed using Agilent 2100 Bioanalyzer system and Qubit DNA HS assay, respectively.
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