Mouse anti tcf4

Protein expression of putative LESC markers ΔNp63, TCF4 and ATP-binding cassette sub-family G member 2 (ABCG2) in hESC-LESCs and hiPSC-LESCs was verified with immunofluorescence stainings, as previously described18 (link). The following primary antibodies were used: goat anti-p63 (Santa Cruz Biotechnology, sc-25039, diluted 1:100), mouse anti-TCF4 (Santa Cruz Biotechnology, sc-166699, diluted 1:400), and mouse anti-ABCG2 (Merck Millipore, MAB4155, diluted 1:200). Their detection was carried out using the following secondary antibodies: Alexa 568-conjugated donkey anti-goat (Molecular Probes, A-11057, diluted 1:800) and Alexa 488-conjugated donkey anti-mouse (Molecular Probes, A-21202, diluted 1:800). Mounting medium containing DAPI (VectaShield, Vector Laboratories Inc., Burlingame, CA) was used for visualization of nuclei. Images were captured using Zeiss LSM 700 confocal microscope (Carl Zeiss, Jena, Germany).

The following antibodies were used: mouse anti- FLAG M2 (Sigma-Aldrich), rabbit anti-HA tag (Abcam), rabbit anti-E-cadherin (Cell Signaling Technology), mouse anti-E-cadherin (DAKO), rabbit anti-ZEB1 (Cell Signaling Technology), rabbit anti-Snail1 (Cell Signaling Technology), rabbit anti-Twist1 (BIORAD), mouse anti-β-catenin (Santa Cruz Biotechnology), mouse anti-TCF4 (Santa Cruz Biotechnology), mouse anti-β-catenin (Sigma-Aldrich), rabbit anti-phospho-STAT3 (Abcam), rabbit anti-STAT3 (Abcam), rabbit anti-galectin-3 (Abcam), goat anti-Trop-2 (R&D), and HRP-conjugated mouse anti-FLAG M2 antibodies (Sigma-Aldrich). Rabbit anti-phospho-Trop-2 antibodies were prepared as described previously (23 (link)).