Rabbit anti cdx2

For immunofluorescence staining on tissue sections, CCSCs, mCCSCs and cCCSCs cells antibodies/antisera used were: mouse anti-Human Nuclei (1:100, Millipore-Merk KGaA, Darmstadt, Germany), mouse anti-BMI1 (1:100, Merk Millipore), rabbit anti-EpCAM (1:50, Cell Signaling, Beverly, MA, USA), rabbit anti-ALDHA1 (1:50, Cell Signaling), rabbit anti-bCatenin (1:50, Cell Signaling), rabbit anti-CD133 (1:100, AbCam, Cambridge, UK), rabbit anti-OLFM4 (1:200, AbCam), rabbit anti-CDX2 (1:100, AbCam), rabbit anti-CK20 (1:100, Abcam), rabbit anti-Lgr5 (1:50, Merk SigmaAldrich), rabbit anti-Laminin (1:400, Merk SigmaAldrich), mouse anti-Vimentin (1:100, Agilent, Santa Clara, CA, USA), mouse anti-HLA-ABC (1:100, Agilent), goat anti-EphA2 (1:50, R&D System, Minneapolis, MN, USA), mouse anti-CXCR4 (1:100, R&D System), rabbit anti-KI67 (1:200, Leica Microsystem, Wetzlar, Germany), mouse anti-CD44 (1:50, BD Biosciences, San Jose, CA, USA), rabbit anti-Wnt5a (1:50, LS Biosciences, Seattle WA, USA), mouse anti-MUC2 (1:50, Novusbio, Littleton, CO, USA). Goat anti mouse AlexaFluor488/546 (1:1000, Thermo Fisher Scientific, Waltham, MA, USA), goat anti rabbit AlexaFluor488 (1:1000, Thermo Fisher Scientific) and donkey anti goat AlexaFluor488 (1:1000, Thermo Fisher Scientific) secondary antibodies were used to visualize the primary antibody staining.