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Rabbit anti cdx2

Manufactured by Abcam
Sourced in United Kingdom, United States

Rabbit anti-CDX2 is a primary antibody that recognizes the CDX2 protein, a transcription factor expressed in the intestinal epithelium. It can be used in various applications such as immunohistochemistry and western blotting to detect and localize the CDX2 protein.

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3 protocols using rabbit anti cdx2

1

Immunofluorescence staining of cancer stem cell markers

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For immunofluorescence staining on tissue sections, CCSCs, mCCSCs and cCCSCs cells antibodies/antisera used were: mouse anti-Human Nuclei (1:100, Millipore-Merk KGaA, Darmstadt, Germany), mouse anti-BMI1 (1:100, Merk Millipore), rabbit anti-EpCAM (1:50, Cell Signaling, Beverly, MA, USA), rabbit anti-ALDHA1 (1:50, Cell Signaling), rabbit anti-bCatenin (1:50, Cell Signaling), rabbit anti-CD133 (1:100, AbCam, Cambridge, UK), rabbit anti-OLFM4 (1:200, AbCam), rabbit anti-CDX2 (1:100, AbCam), rabbit anti-CK20 (1:100, Abcam), rabbit anti-Lgr5 (1:50, Merk SigmaAldrich), rabbit anti-Laminin (1:400, Merk SigmaAldrich), mouse anti-Vimentin (1:100, Agilent, Santa Clara, CA, USA), mouse anti-HLA-ABC (1:100, Agilent), goat anti-EphA2 (1:50, R&D System, Minneapolis, MN, USA), mouse anti-CXCR4 (1:100, R&D System), rabbit anti-KI67 (1:200, Leica Microsystem, Wetzlar, Germany), mouse anti-CD44 (1:50, BD Biosciences, San Jose, CA, USA), rabbit anti-Wnt5a (1:50, LS Biosciences, Seattle WA, USA), mouse anti-MUC2 (1:50, Novusbio, Littleton, CO, USA). Goat anti mouse AlexaFluor488/546 (1:1000, Thermo Fisher Scientific, Waltham, MA, USA), goat anti rabbit AlexaFluor488 (1:1000, Thermo Fisher Scientific) and donkey anti goat AlexaFluor488 (1:1000, Thermo Fisher Scientific) secondary antibodies were used to visualize the primary antibody staining.
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2

Plasmid Cloning and Antibody Validation

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The inserts of plasmids pcs2-UTX and pcs2-ZSCAN4D used in this study were PCR-amplified from the cDNA of NIH-3T3 cells and 2-cell mouse embryos, respectively. The PCR products were cloned into the Xho I and Xba I (Thermo, USA) sites downstream of the Myc ectopic tag. The antibodies covered were rabbit anti-UTX (Millipore, USA, for IF and WB), rabbit anti-H3K27me3 (Abcam, USA, for IF), mouse anti-Myc (Santa Cruz, USA, for IF), rabbit anti-ZSCAN4 (Millipore, for IF), rabbit anti-CDX2 (Abcam, for IF), mouse anti-NANOG (Abcam, for IF), rabbit α-tubulin (Proteintech, USA, for WB), Alexa Fluor 594 and Alexa Fluor 488 (Thermo, for IF), anti-UTX (KDM6A) (Abcam, for ChIP), anti-IgG (Abcam, for ChIP).
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3

Multimarker Immunofluorescence Characterization

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We performed antibody staining of the differentiated cells according to standard protocols. The following primary antibodies were used: Rabbit anti-CDX2 (Abcam), Mouse anti-CDX2 (BioGenex), Goat anti-SOX2 (Santa Cruz), Rabbit anti-E-Cadherin (Cell signaling), Rabbit anti-Vimentin (cell signaling), Rabbit anti-FoxF1 (abcam), Rabbit anti-PDGFRB (cell signaling), Rabbit anti-LEF1 (cell signaling), Rabbit anti-Hand1 (Novus Biologicals) and Mouse anti-APLNR (R&D Systems). We stained the nuclei by using Hoechst 33258 (Sigma). The primary antibodies were detected by fluorescent-conjugated secondary antibodies from Jackson Immuno Research Laboratories. We collected the images by using the Zeiss LSM 780 confocal microscope and processed the images with Adobe Illustrator.
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