High viability cryohepatocyte recovery kit

Cryopreserved human hepatocytes from three donors (#1: HMC 514, #2: HFC920, #3: HMC1034), the High Viability Cryohepatocyte Recovery Kit, Hepatocyte Culture Media Kit, Matrigel matrix, and collagen I-coated 24-well plates were obtained from Corning Life Sciences (Woburn, MA, USA). GlutaMAX-I supplement was purchased from Gibco (Grand Island, NY, USA). Cryopreserved HepaRG cells, HepaRG Thaw, Plate, and General Purpose Medium Supplement, and HepaRG Induction Medium Supplement were obtained from Biopredic (Rennes, France). Calcitriol, omeprazole, 6-(4-chlorophenyl)imidazo[2,1-b] [1,3]thiazole-5-carbaldehyde O-3,4-dichlorobenzyl) oxime (CITCO), rifampin, bupropion, and William’s Medium E were purchased from Sigma-Aldrich (St. Louis, MO, USA). Superscript III First-Strand Synthesis SuperMix, TRIzol, and diethyl pyrocarbonate (DEPC)-treated water were obtained from Invitrogen (Carlsbad, CA, USA). Quantitative polymerase chain reaction (qPCR) reagents were purchased from Applied Biosystems (Foster City, CA, USA). Dimethyl sulfoxide (DMSO) was obtained from Merck (Merck-Millipore, Darmstadt, Germany). All other chemicals were of reagent grade or better and were used without further purification.