Integrin α1

Manufactured by Abcam

Sourced in United States

Integrin α1 is a cell surface receptor that mediates cell-extracellular matrix interactions. It is a member of the integrin family of proteins and is involved in various cellular processes, including cell adhesion, migration, and signaling.

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Lab products found in correlation

Integrin α2, by Abcam (1 mentions) Integrin β1, by Abcam (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Odyssey scanner, by LI COR (1 mentions) Adaptin γ, by BD (1 mentions) α tubulin, by Merck Group (1 mentions) Gapdh, by Thermo Fisher Scientific (1 mentions) Hrp conjugated antibodies, by Jackson ImmunoResearch (1 mentions)

2 protocols using integrin α1

Evaluating Integrin Expression in HUVECs

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HUVECs were treated with SMCM, SMC-NC, or iSMC-NC for 24 h, then total proteins extracted using RIPA buffer containing phenylmethanesulfonylfluoride (PMSF). After a 30-min incubation on ice, protein lysates were centrifuged at 13,000 rpm for 15 min and supernatants collected. Protein concentrations were measured using the BCA Protein Assay Kit (Pierce, Rockford, IL, USA). Ten micrograms of each protein sample were subjected to 8% SDS-PAGE and transferred to PVDF membranes, which were then blocked with 5% BSA in TBS containing 0.1% Tween-20 at room temperature for 1 h and incubated with primary antibodies diluted in blocking buffer at 4°C overnight. After washing with PBS containing 0.1% Tween, secondary antibodies were added for incubation at room temperature for 1 h. Western blot images were captured using a LI-COR Odyssey Scanner. Primary antibodies to detect human VEGF-A (Abcam), integrin α1 (Abcam), integrin α2 (Abcam), integrin β1 (Abcam), and GAPDH (Cell Signaling Technology, Danvers, MA, USA) were used.

Liu P., Shi Y., Fan Z., Zhou Y., Song Y., Liu Y., Yu G., An Q, & Zhu W. (2018). Inflammatory Smooth Muscle Cells Induce Endothelial Cell Alterations to Influence Cerebral Aneurysm Progression via Regulation of Integrin and VEGF Expression. Cell Transplantation, 28(6), 713-722.

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NEDD9 Immunoprecipitation and Western Blot

Cited 1 time

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Western blotting was done as outlined in previous reports [1 ]. The primary antibodies included mouse anti-NEDD9 mAb (2G9) [1 ], -integrin β1, - pAP2 µ2 (Cell Signaling Technology), -integrin α1 (Abcam), -pTyr14/caveolin1, -caveolin1 (Santa Cruz Biotechnology), -GAPDH (ThermoFisher Scientific), -α-tubulin (Sigma-Aldrich), -Ras (EMD Millipore), -EGFR (eBioscience), - Transferin receptor, -adaptin-α, and -adaptin-γ (BD Bioscience). The secondary HRP-conjugated antibodies (Jackson Immuno Research Labs) were followed by using a chemiluminescent HRP Detection Reagent (Denville Scientific). The bands were digitized and quantified using a digital documentation and image analysis software (Syngene). For immunoprecipitation, the 2×10⁶ cells were lysed in PTY buffer and incubated with 1µg of anti-NEDD9 mAb (2G9) or the control IgG overnight at +4°C as previously described [27 (link)].

Kozyulina P.Y., Loskutov Y.V., Kozyreva V.K., Rajulapati A., Ice R.J., Jones B.C, & Pugacheva E.N. (2014). Pro-metastatic NEDD9 regulates individual cell migration via caveolin-1-dependent trafficking of integrins. Molecular cancer research : MCR, 13(3), 423-438.

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