Terminal deoxynucleotidyltransferase mediated dutp nick end labeling tunel

Manufactured by Roche

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Terminal Deoxynucleotidyltransferase-mediated dUTP Nick End Labeling (TUNEL) is a method used to detect and quantify apoptosis, or programmed cell death, in cells. The assay utilizes terminal deoxynucleotidyl transferase (TdT) to label the free 3'-hydroxyl termini of DNA strand breaks that occur during apoptosis.

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Lab products found in correlation

Dihydroethidium dhe, by Thermo Fisher Scientific (3 mentions) Antibody for β actin, by Merck Group (2 mentions) Antibody against grp 78, by Bioworld Technology (2 mentions) Tunicamycin, by Merck Group (2 mentions) N acetyl l cysteine, by Merck Group (2 mentions) α actinin, by Cell Signaling Technology (1 mentions) Phospho eif2α, by Cell Signaling Technology (1 mentions) Total eif2α, by Cell Signaling Technology (1 mentions) 4 4 diisothiocyanatostilbene 2 2 disulfonic acid disodium salt hydrate, by Merck Group (1 mentions) β catenin, by Cell Signaling Technology (1 mentions) Trypan blue t 6146, by Merck Group (1 mentions) Melatonin, by Merck Group (1 mentions) Luzindole, by Merck Group (1 mentions) Live dead kit, by Thermo Fisher Scientific (1 mentions) Ferric ammonium citrate, by Merck Group (1 mentions) Calcein am, by Yeasen (1 mentions) Stem cell medium, by STEMCELL (1 mentions) Lamp2, by GeneTex (1 mentions) Z devd fmk, by Santa Cruz Biotechnology (1 mentions) Anti rabbit secondary antibody, by Merck Group (1 mentions)

Close spelling variants of this product

Terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling (TUNEL) assay kit (4 citations)

5 protocols using terminal deoxynucleotidyltransferase mediated dutp nick end labeling tunel

Cellular Mechanisms of Tunicamycin-Induced Stress

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Tunicamycin, 4,4′-Diisothiocyanatostilbene-2,2′-disulfonic acid disodium salt hydrate (DIDS), 4-(2-butyl-6,7-dichlor-2-cyclopentyl- indan-1-on-5-yl)oxybutyric acid (DCPIB), 5-bromo-2-deoxyuridine (BrdU), 3-(4,5- dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), N-acetyl-l-cysteine (NAC) and antibody for β-actin were purchased from Sigma-Aldrich Corporation (St. Louis, MO, USA). sFRP was purchased from R&D Systems (Minneapolis, MN, USA). Antibody against GRP78 was from Bioworld Technology (St. Louis Park, MN, USA). Antibodies against CHOP and ATF4 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Phospho-eIF2α, total eIF2α, β-catenin and α-actinin were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibody against Lamin B was from Boster (Wuhan, China). Dihydroethidium (DHE) was from Molecular Probes (Eugene, OR, USA). Terminal Deoxynucleotidyltransferase-mediated dUTP Nick End Labeling (TUNEL) was from Roche Applied Science (Sandhofer Strasse, Mannheim, Deutschland).

Shen M., Wang L., Wang B., Wang T., Yang G., Shen L., Wang T., Guo X., Liu Y., Xia Y., Jia L, & Wang X. (2014). Activation of volume-sensitive outwardly rectifying chloride channel by ROS contributes to ER stress and cardiac contractile dysfunction: involvement of CHOP through Wnt. Cell Death & Disease, 5(11), e1528-.

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Melatonin and Stem Cell Viability

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Melatonin, ferric ammonium citrate (FAC) and luzindole were purchased from Sigma (St, Louis. Mo. USA). Melatonin, FAC and luzindole were dissolved in dimethyl sulfoxide (DMSO) and the final culture concentration of DMSO was ≤ 0.1%. Other chemicals were all purchased from Sigma (St, Louis. Mo. USA). Stem cell medium was purchased from Stem Cell (Canada). Calcein-AM (40719ES60) was purchased from Shanghai YEASEN Biotechnology. Trypan blue (T-6146) was purchased from Sigma (St, Louis. Mo. USA). Live/Dead Kit (1736918) was purchased from Life technologies. Terminal Deoxynucleotidyl Transferase-Mediated dUTP Nick-End Labeling (TUNEL) was purchased from Roche Company (Roche, Germany). PI/Hoechst staining (CA1120) was purchased from Solarbio (Beijing Solarbio Science & Technology, China).

Yang F., Li Y., Yan G., Liu T., Feng C., Gong R., Yuan Y., Ding F., Zhang L., Idiiatullina E., Pavlov V., Han Z., Ma W., Huang Q., Yu Y., Bao Z., Wang X., Hua B., Du Z., Cai B, & Yang L. (2017). Inhibition of iron overload-induced apoptosis and necrosis of bone marrow mesenchymal stem cells by melatonin. Oncotarget, 8(19), 31626-31637.

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Evaluating Autophagy and Apoptosis

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DCPIB, NAC, 5-bromo-2-deoxyuridine (BrdU), MTT, 3-MA, antimouse and anti-rabbit secondary antibodies were purchased from Sigma-Aldrich (St. Louis, USA); LC3B, Beclin-1, GAPDH and LAMP2 primary antibodies were purchased from Genetex (Abcam, England); Antibodies against Beclin1 was obtained from Sigma-Aldrich (St. Louis, USA). Dihydroethidium (DHE) was from Molecular Probes (Eugene, OR, USA). LC3II primary antibody and Z-DEVD-FMK were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA); Terminal Deoxynucleotidyltransferase- mediated dUTP Nick End Labeling (TUNEL) was from Roche Applied Science (SandhoferStrasse, Mannheim, Deutschland).

Xia Y., Liu Y., Xia T., Li X., Huo C., Jia X., Wang L., Xu R., Wang N., Zhang M., Li H, & Wang X. (2016). Activation of volume-sensitive Cl− channel mediates autophagy-related cell death in myocardial ischaemia/reperfusion injury. Oncotarget, 7(26), 39345-39362.

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Tunicamycin-Induced Endoplasmic Reticulum Stress

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Tunicamycin, baicalin, 5-bromo-2-deoxyuridine (BrdU), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Lactate dehydrogenase (LDH), L-NAME (NOS inhibitor), and antibody for β-actin were purchased from Sigma-Aldrich Corporation (St. Louis, MO, USA). Antibody against GRP 78 was from Bioworld Technology (St. Louis Park, MN, USA). Antibodies against CHOP and eNOS were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against caspase-3 and phospho eNOS were from Cell Signaling Technology (Danvers, MA, USA). DAF-FM was from Molecular Probes (Eugene, OR, USA). Terminal Deoxynucleotidyltransferase-mediated dUTP Nick End Labeling (TUNEL) was from Roche Applied Science (Sandhofer Strasse, Mannheim, Deutschland).

Shen M., Wang L., Yang G., Gao L., Wang B., Guo X., Zeng C., Xu Y., Shen L., Cheng K., Xia Y., Li X., Wang H., Fan L, & Wang X. (2014). Baicalin Protects the Cardiomyocytes from ER Stress-Induced Apoptosis: Inhibition of CHOP through Induction of Endothelial Nitric Oxide Synthase. PLoS ONE, 9(2), e88389.

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Cellular Signaling and Viability Assays

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D-(+)-Glucose, 4,4′-Diisothiocyanatostilbene-2,2′-disulfonic acid disodium salt hydrate (DIDS), 4-(2-butyl-6,7-dichlor-2-cyclopentyl-indan-1-on-5-yl) oxybutyric acid (DCPIB), 5-bromo-2-deoxyuridine (BrdU), 3-(4,5-dimethylthiazol-2-yl)-2, 3-methyladenine(3-MA), bafilomycin A1 (BAF), 5-diphenyltetrazolium bromide (MTT), n-acetyl-l-cysteine (NAC), and antibody for β-actin, paraformaldehyde, were purchased from Sigma-Aldrich Corporation (St. Louis, MO, USA). Cell Signaling Technology: Anti-p62 c-terminus/SQSTM1 antibody(GP-62C), LC3B(2775). Antibody against pro-caspase-3(2912). 3-methyladenine (Sigma, M9281) in DMEM (HyClone, 11966), and bafilomycin A1 (LC Laboratories, B-1080) in dimethylsulfoxide (DMSO; Sigma, 472301). Each drug was added to the medium to final concentration as described in each experiment. Dihydroethidium (DHE) was from Molecular Probes (Eugene, OR, USA).Terminal deoxynucleotidyltransferase-mediated dUTP Nick End Labeling (TUNEL) was from Roche Applied Science (Sandhofer Strasse, Mannheim, Deutschland). N-(Ethoxycarbonylmethyl)-6-methoxyquinolinium bromide(MQAE) was purchased from Invitrogen Corporation(USA; E-3101) Molecular Probes, Inc. Cell apoptosis assay by Annexin-V-FITC(fluorescein isothiocyanate)/propidium iodide (PI) staining).

Wang L., Shen M., Guo X., Wang B., Xia Y., Wang N., Zhang Q., Jia L, & Wang X. (2017). Volume-sensitive outwardly rectifying chloride channel blockers protect against high glucose-induced apoptosis of cardiomyocytes via autophagy activation. Scientific Reports, 7, 44265.

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