Leukotriene e4 elisa kit

Nasal secretions were analysed in serially diluted samples for content of LTB 4 , LTE 4 Prostaglandin (PG) D 2 , PGE 2 and 15(S)-hydroxy eicosatetraenoic acid (15-(S)HETE), using specific ELISA kits (Cayman Chemical, Ann Arbor, MI, USA). All analyses were performed according to manufactures instructions and measured spectrophotometrically at 410 nm with the infinite M200 Pro-microplate reader (Tecan, Switzerland). The lower detection limits range from 5.0 to 58.9 pg/ml for the different ELISA assays.
Analysis of urinary LTE 4 was performed in serially diluted samples with an enzyme linked immunosorbent Leukotriene E 4 ELISA kit (Cayman Chemical, Ann Arbor, MI, USA) essentially as previously described [32] . The specificity of the rabbit polyclonal antiserum was LTE 4 (100%) and for LTD 4 and LTC 4 (<0.01%). Analysis of 11β-PGF 2 α was performed with a similar protocol as previously described [33] using a commercially available 11β-PGF 2α ELISA kit (Cayman Chemical Company, Ann Arbor, MI, USA). The antibody cross-reacted with 11β-PGF 2α (100%), 2,3-dinor-9α,11β-PGF 2 (10%), 11β-13,14 dihydro-15-keto-PGF 2α (0.5%). All urine samples were subjected to creatinine analysis using a colorimetric assay (Cayman Chemical, Ann Arbor, MI, USA) and results are expressed as ng/mmol of creatinine for correction of diuresis variations.