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96 well black sided clear bottom costar plate

Manufactured by Corning
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The 96-well black sided clear bottom costar plate is a laboratory equipment product designed for various applications in scientific research. It features a 96-well format with black sides and a clear bottom, providing both optical clarity and light-blocking capabilities. The core function of this product is to serve as a versatile platform for various cell-based assays, absorbance measurements, and other analytical techniques that require a multi-well microplate format.

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Lab products found in correlation

Dithiothreitol (dtt), by Thermo Fisher Scientific (2 mentions) Heparin, by Thermo Fisher Scientific (2 mentions) Thioflavin t, by Thermo Fisher Scientific (2 mentions) Heparin, by MP Biomedicals (2 mentions) Synergy h1, by Agilent Technologies (2 mentions) Thioflavin t, by Merck Group (2 mentions)

2 protocols using 96 well black sided clear bottom costar plate

1

Tau Aggregation Inhibition Assay

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Recombinant human P301L tau was dialyzed into 100 mM sodium acetate buffer pH 7.4 overnight. Samples consisting of 20 μM P301L tau and 2x the indicated concentration of compound were incubated at room temperature for 1 hour prior to starting the assay. Using a master mix of 2x concentrated heparin (MP Biomedicals, Santa Anna, CA), Thioflavin T (Sigma-Aldrich, St. Louis, MO), and dithiothreitol (ThermoFisher Scientific, Waltham, MA), samples were diluted to a final concentration of 10 μM P301L tau, 2.5 μM heparin, 10 μM Thioflavin T, the compounds at indicated concentrations or DMSO, and 2 mM dithiothreitol in 100 mM sodium acetate at pH 7.4. A total of 200 μL was added to each well of a low binding 96-well black sided clear bottom costar plate (Corning, Corning, NY). Each plate was incubated at 37 °C, and fluorescence intensity (440 excitation, 482 emission) was measured over a 5-day period using a BioTek (Winooski, VT) Synergy H1 microplate reader.
Hill S.E., Beaulieu-Abdelahad D., Lemus A., Webster J.M., Ospina S.R., Darling A.L., Martin M.D., Patel S., Bridenstine L., Swonger R., Paul S., Blackburn R., Calcul L., Dickey C.A., Leahy J.W, & Blair L.J. (2023). Benzothiazole Substitution Analogs of Rhodacyanine Hsp70 Inhibitors Modulate Tau Accumulation. ACS chemical biology, 18(5).
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2

Tau Aggregation Inhibition Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant human P301L tau was dialyzed into 100 mM sodium acetate buffer pH 7.4 overnight. Samples consisting of 20 μM P301L tau and 2x the indicated concentration of compound were incubated at room temperature for 1 hour prior to starting the assay. Using a master mix of 2x concentrated heparin (MP Biomedicals, Santa Anna, CA), Thioflavin T (Sigma-Aldrich, St. Louis, MO), and dithiothreitol (ThermoFisher Scientific, Waltham, MA), samples were diluted to a final concentration of 10 μM P301L tau, 2.5 μM heparin, 10 μM Thioflavin T, the compounds at indicated concentrations or DMSO, and 2 mM dithiothreitol in 100 mM sodium acetate at pH 7.4. A total of 200 μL was added to each well of a low binding 96-well black sided clear bottom costar plate (Corning, Corning, NY). Each plate was incubated at 37 °C, and fluorescence intensity (440 excitation, 482 emission) was measured over a 5-day period using a BioTek (Winooski, VT) Synergy H1 microplate reader.
Stadler J., Trockfeld J., Schmalix W.A., Brill T., Siewert J.R., Greim H, & Doehmer J. (1994). Inhibition of cytochromes P4501A by nitric oxide.. Proceedings of the National Academy of Sciences of the United States of America, 91(9), 3559-3563.
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