For separating prostate stromal cells, dissociated murine prostate cells were prepared as described previously (15 (link)) and stained with Pacific blue-anti CD31, CD45 and Ter119 antibodies (eBioscience, San Diego, CA), PE-anti Sca-1 antibody (eBioscience, San Diego, CA), Alexa 647-anti CD49f antibody (Biolegend, San Diego, CA). Prostate stromal cells are CD31−CD45−Ter119−Sca-1+CD49f− (48 (link)). For detecting MDSC cells, dissociated murine prostate cells were stained with Pacific blue-anti CD11b (eBioscience, San Diego, CA), and PE-Cy7-anti Gr-1 antibodies (Biolegend, San Diego, CA). FACS analyses and sorting were performed by using the BD LSR Fortessa and Aria II, respectively (BD Biosciences, San Jose, CA).
Pe anti sca 1 antibody
Manufactured by Thermo Fisher Scientific
The PE-anti Sca-1 antibody is a laboratory reagent used to detect the presence of the Sca-1 (Stem Cell Antigen-1) protein in samples. It is conjugated with the fluorescent dye Phycoerythrin (PE) to enable visualization and quantification of Sca-1-expressing cells through flow cytometry or other fluorescence-based techniques.
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2 protocols using pe anti sca 1 antibody
1
Isolation of Murine Prostate Stromal Cells
2
Isolation of Murine Prostate Stromal Cells
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For separating prostate stromal cells, dissociated murine prostate cells were prepared as described previously (15 (link)) and stained with Pacific blue-anti CD31, CD45 and Ter119 antibodies (eBioscience, San Diego, CA), PE-anti Sca-1 antibody (eBioscience, San Diego, CA), Alexa 647-anti CD49f antibody (Biolegend, San Diego, CA). Prostate stromal cells are CD31−CD45−Ter119−Sca-1+CD49f− (48 (link)). For detecting MDSC cells, dissociated murine prostate cells were stained with Pacific blue-anti CD11b (eBioscience, San Diego, CA), and PE-Cy7-anti Gr-1 antibodies (Biolegend, San Diego, CA). FACS analyses and sorting were performed by using the BD LSR Fortessa and Aria II, respectively (BD Biosciences, San Jose, CA).
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