Anti p nf κb p65 antibody

Manufactured by Cell Signaling Technology

Sourced in United States, China

The Anti-p-NF-κB-p65 antibody is a laboratory research tool that specifically binds to and detects the phosphorylated form of the NF-κB p65 subunit. This antibody can be used in various experimental techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the activation and regulation of the NF-κB signaling pathway.

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P-NF-κB (p65) antibody Anti-p-NF-κB p65 Anti-NF-κB p65 antibody P-NF-κB p65 NF-κB p65 antibody Anti-NF-κB p65 P-NF-κB antibody Anti-p-NF-κB NF-κB p65 Anti-NF-κB antibody

2 protocols using anti p nf κb p65 antibody

Western Blot Analysis of Signaling Proteins

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The whole-cell lysate was generated with radio-immunoprecipitation assay (RIPA) lysis buffer, and the protein concentration was measured by Bio-Rad (Hercules, CA, USA) protein assay following the manufacturer's instructions. The proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrotransferred onto polyvinylidene fluoride (PVDF) membranes, and the blots were blocked and incubated overnight at 4°C with the appropriate primary antibody. The following antibodies which obtained from Cell Signaling Technologies (Beverly, MA, USA) were used in this study: anti-SYK antibody (#80460), anti-NF-κB p65 antibody (#8242), anti-IκBα antibody (#4814), anti-ERK1/2 antibody (#4696), anti-p38 antibody (#8690), anti-p-SYK antibody (#2710), anti-p-NF-κB-p65 antibody (#3033), anti-p-IκBα antibody (#2859), anti-p-ERK1/2 antibody (#4370), and anti-p-p38 antibody (#4511). Both the anti-TOSO antibody (#ab56487) and anti-BCL-2 antibody (#ab13-8800) were obtained from Abcam, Cambridge, UK. The blots were then incubated with a specific HRP-conjugated secondary antibody (CST#7074 and 7076, Beverly). Immunodetection was performed by enhanced chemiluminescence.

Zhang Y.R., Yu Z., Xiong W.J., Liu X.X., Liu H.M., Cui R., Wang Q., Chen W.M., Qiu L.G, & Yi S.H. (2020). TOSO interacts with SYK and enhances BCR pathway activation in chronic lymphocytic leukemia. Chinese Medical Journal, 133(17), 2090-2097.

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Mitochondrial KATP Channel Modulation

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(NRG), 5,5′,6,6′-tetrachloro-1,1′,3,3′tetraethyl-imidacarbocyanine iodide (JC-1), Hoechst 33258, STZ and pyrrolidine dithiocarbamate (PDTC, a specific inhibitor of NF-κB pathway) were purchased from Sigma-Aldrich. Diazoxide (DZ, a mitochondrial K ATP channel opener), pinacidil (Pin, a non-selective K ATP channel opener), 5-hydroxydecanoic acid (5-HD, a mitochondrial K ATP channel blocker) and glibenclamide (Gli, a non-selective K ATP channel blocker) were purchased from Cayman Chemical. The cell counter kit-8 (CCK-8) was supplied by Dojindo Lab (Kumamoto, Japan). Fetal bovine serum (FBS) and DMEM medium were obtained from Gibco BRL; anti-kir6.1 (R-14) was purchased from Santa Cruz Biotechnology; anti-p-NF-κB p65 antibody, anti-p65 antibody anticaspase-3 antibody, anti-bax antibody and anti-bcl-2 antibody were purchased from Cell Signaling Technology; HRPconjugated secondary antibody and BCA protein assay kit were obtained from KangChen Bio-tech, Inc. (Shanghai, China). Enhanced chemiluminescence (ECL) solution was purchased from KeyGen Biotech (Nanjing, China).

You Q., Wu Z., Wu B., Liu C., Huang R., Yang L., Guo R., Wu K, & Chen J. (2016). Naringin protects cardiomyocytes against hyperglycemia-induced injuries in vitro and in vivo. The Journal of endocrinology, 230(2).

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