Pcdna3.1 drp1

Manufactured by Addgene

pCDNA3.1-Drp1 is a plasmid vector that contains the coding sequence for the Dynamin-related protein 1 (Drp1). Drp1 is a key regulator of mitochondrial fission. The vector allows for the expression of Drp1 in mammalian cell lines.

Automatically generated - may contain errors

Lab products found in correlation

Megfp c1, by Addgene (2 mentions) Pmig pp2aa, by Addgene (2 mentions) Pmig pp2ab, by Addgene (2 mentions)

Close spelling variants of this product

PcDNA3.1

2 protocols using pcdna3.1 drp1

Plasmid Cloning and Lentiviral Production

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pMIG-PP2Aa (#10884), pMIG-PP2Ab (#13804) and pcDNA3.1-Drp1 (#34706) plasmids were purchased from Addgene and subcloned into mEGFP-C1 (#54759) and pCMV-Myc-3B vectors. RalA^WT, RalA^G23V and RalA^S28N plasmids were subcloned into a pLVX vector with 3× Flag tag for lentiviral production.

Xia W., Veeragandham P., Cao Y., Xu Y., Rhyne T.E., Qian J., Hung C.W., Zhao P., Jones Y., Gao H., Liddle C., Yu R.T., Downes M., Evans R.M., Rydén M., Wabitsch M., Wang Z., Hakozaki H., Schöneberg J., Reilly S.M., Huang J, & Saltiel A.R. (2024). Obesity causes mitochondrial fragmentation and dysfunction in white adipocytes due to RalA activation. Nature Metabolism, 6(2), 273-289.

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Molecular Cloning of RalA Variants

Check if the same lab product or an alternative is used in the 5 most similar protocols

pMIG-PP2Aa (#10884), pMIG-PP2Ab (#13804), and pcDNA3.1-Drp1 (#34706) plasmids were purchased from Addgene and subcloned into mEGFP-C1 (#54759) and pCMV-Myc-3B vectors. RalA^WT, RalA^G23V, and RalA^S28N plasmids were subcloned into a pLVX vector with 3x Flag tag for lentiviral production.

xia W., Veeragandham P., Cao Y., Xu Y., Rhyne T., Qian J., Hung C.W., Zhao P., Jones Y., Gao H., Liddle C., Yu R., Downes M., Evans R., Ryden M., Wabitsch M., Reilly S., Huang J, & Saltiel A. (2023). Obesity-dependent increase in RalA activity disrupts mitochondrial dynamics in white adipocytes. Research Square.

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