Anti sca 1 d7

The lung, liver, spleen, kidney, and heart were extracted after mice were perfused with 1X PBS via the right atrium. For the heart, we used collagenase IV (10 mg/mL) as a digestive enzyme. For the spleen, no digestive enzymes were needed. The other tissues used collagenase type I, collagenase XI, and hyaluronidase. For the heart, we used collagenase IV. The cell suspensions were filtered through a 70-μm sterile nylon mesh cell strainer, washed with 1X PBS, and transferred to Eppendorf tubes. Fc receptors were blocked using TruStain fcX™ anti-mouse CD16/32 (BioLegend). The following antibodies were used: clone 6D5, 17A2, N418, M1/70, FA11, 30-F11, and 390. We also used anti-TER-119 (TER-119, BioLegend), anti-Annexin V (BioLegend), DAPI for nucleic acid staining (Sigma-Aldrich), PE anti-mCD47 (miap301, BioLegend), CD326 (G8.8, BioLegend), anti-Sca-1 (D7, BioLegend), anti-CD24 (M1/69, BD Biosciences), anti-CD271 (ME20.4, Invitrogen), and Monorab™ Rabbit Anti-Camelid VHH antibody, mAb (96A3F5, GenScript). Each stain was added at a 1:200 dilution to the cell suspension. Flow gating is shown (SI Appendix, Figs. S18 and S19). When Ai14 mice were used to quantify delivery, we also used PBS-treated Ai14 mice to control for flow gating.