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Overexpression and knockdown lentiviruses for lhpp nm 022126.4

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Overexpression and knockdown lentiviruses for LHPP (NM_022126.4) are genetic tools designed to modulate the expression of the LHPP gene. LHPP is a protein-coding gene that plays a role in cellular processes. These lentiviral vectors can be used to increase or decrease the expression of LHPP in cell lines or other experimental systems.

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Lab products found in correlation

Control lentivirus, by Genechem (2 mentions) Puromycin, by Merck Group (2 mentions)

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Lentivirus (335 citations) Overexpression lentivirus (4 citations) Knockdown lentivirus (3 citations)

2 protocols using overexpression and knockdown lentiviruses for lhpp nm 022126.4

1

Lentiviral Overexpression and Knockdown

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Overexpression and knockdown lentiviruses for LHPP (NM_022126.4), as well as control lentivirus, were purchased from GeneChem Corporation (Shanghai, China). Transfection was performed according to the manufacturer’s instructions. Puromycin (2 μg/ml, Sigma) was used to select stable clones for at least 1 week. At the indicated time points, the cells were harvested for mRNA and protein analysis as well as for other assays.
Lin J.X., Lian N.Z., Gao Y.X., Zheng Q.L., Yang Y.H., Ma Y.B., Xiu Z.S., Qiu Q.Z., Wang H.G., Zheng C.H., Li P., Xie J.W., Lu J., Chen Q.Y., Cao L.L., Lin M., Wang J.B, & Huang C.M. (2022). m6A methylation mediates LHPP acetylation as a tumour aerobic glycolysis suppressor to improve the prognosis of gastric cancer. Cell Death & Disease, 13(5), 463.
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2

Lentivirus-mediated LHPP modulation

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Overexpression and knockdown lentiviruses for LHPP (NM_022126.4) as well as control lentivirus were purchased from GeneChem Corporation (Shanghai, China). Transfection was performed according to the manufacturer's instructions. Puromycin (2 μg/ml, Sigma) was used to select stable clones for at least 1 week. At the indicated time points, the cells were harvested for mRNA and protein analysis as well as for other assays.
Lin J., Lian N., Gao Y., Qiu Q., Wang H., Zheng Q., Yang Y., Ma Y., Zheng C., Li P., Xie J., Lü J., Chen Q., Cao L., Lin M., Wang J., & Huang C. (2021). m6A Methylation Mediates LHPP Acetylation as a Tumour Aerobic Glycolysis Suppressor to Improve the Prognosis of Gastric Cancer.
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