Gp susceptibility card ast p592

ARGs were identified in the E. faecium genomes using the Genome Annotation Service in PATRIC by deploying the k-mer-based ARG detection method, which use PATRIC’s curated collection of representative antibiotic-resistance variants [19 (link)] and assigns to each ARG functional annotation, broad mechanism of antibiotic resistance, drug class and specific antibiotic it confers resistance to.
ARG screening results were validated using minimum inhibitory concentration (MIC) and antibiotic-sensitivity tests. Bacterial suspensions equivalent to 0.5 McFarland turbidity were prepared and 3 ml suspension equivalent to 10⁷ c.f.u. ml⁻¹ was subjected to AST. The test was performed using the GP susceptibility card AST-P592 (bioMérieux) and the VITEK 2 system (version 9.02), according to the manufacturer’s guidelines. MIC interpretation was performed according to the recommendations of the Clinical and Laboratory Standards Institute (CLSI). The antibiotic-sensitivity test was carried out on Muller Hinton agar (MHA; HiMedia) plates containing antibiotic discs. The plates were incubated at 37 °C for 24 h and the results were expressed in millimetres of inhibition. The isolates were classified as susceptible (S), intermediate (I) or resistant (R) based on CLSI recommendations [20 ].