Taxus wallichiana var. chinensis (Pilg.) Florin was purchased from the pharmacy in the First Affiliated Hospital of Zhejiang Chinese Medical University (Hangzhou, China), which was manufactured by the Ningbo Taikang Yew Biological Engineering Company (Batch No. 100513). Human lung cancer (LC) cell lines, including A549, NCI-H358, HCC827, and MRC-5, were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). They were cultured by 0.25% trypsin digestion in the mediums of F-12K (21127-022, GIBCO, Hangzhou, China), RPMI-1640 (C11875500BT, GIBCO, Hangzhou, China), and MEM (41500034, GIBCO, Hangzhou, China) for 1–2 mins at 37°C in a humidified atmosphere consisting of 5% CO2 separately, where NCI-H358 and HCC827 were both cultured in RPMI-1640 medium. The antibodies included N-cadherin (1:3000, ab98952, Abcam), E-cadherin (1:2000, 20874-1-AP, Proteintech), vimentin (1:2000, ab92547, Abcam), p-JAK (1:500, ab32101, Abcam), p-STAT3 (1:500, ab76315, Abcam), JAK (1:1000, 3230, CST), STAT3 (1:1000, 06-596, Merck), and GAPDH (1:5000, 70-Mab5465-040, MultiSciences).
70 mab5465 040
Manufactured by MultiSciences Biotech
The 70-Mab5465-040 is a laboratory equipment product manufactured by MultiSciences Biotech. It is a compact and versatile device designed for precise liquid handling and sample preparation tasks in a research or clinical laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Ab98952, by Abcam (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 c11875500bt, by Thermo Fisher Scientific (1 mentions)
Nci h358, by Thermo Fisher Scientific (1 mentions)
Hcc827, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Ab92547, by Abcam (1 mentions)
Ab76315, by Abcam (1 mentions)
Ab32101, by Abcam (1 mentions)
Anti sox2 af2018, by R&D Systems (1 mentions)
Rag0072, by MultiSciences Biotech (1 mentions)
Anti brachyury, by Santa Cruz Biotechnology (1 mentions)
Gb13102 1, by Wuhan Servicebio Technology (1 mentions)
Anti stat3, by Cell Signaling Technology (1 mentions)
Anti pdgf d, by Santa Cruz Biotechnology (1 mentions)
Anti kdr, by Cell Signaling Technology (1 mentions)
Anti phospho erk, by Cell Signaling Technology (1 mentions)
Anti erk, by Cell Signaling Technology (1 mentions)
Gar0072, by MultiSciences Biotech (1 mentions)
Gam0072, by MultiSciences Biotech (1 mentions)
2 protocols using 70 mab5465 040
1
Taxus wallichiana var. chinensis Lung Cancer Protocol
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Taxus wallichiana var. chinensis (Pilg.) Florin was purchased from the pharmacy in the First Affiliated Hospital of Zhejiang Chinese Medical University (Hangzhou, China), which was manufactured by the Ningbo Taikang Yew Biological Engineering Company (Batch No. 100513). Human lung cancer (LC) cell lines, including A549, NCI-H358, HCC827, and MRC-5, were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). They were cultured by 0.25% trypsin digestion in the mediums of F-12K (21127-022, GIBCO, Hangzhou, China), RPMI-1640 (C11875500BT, GIBCO, Hangzhou, China), and MEM (41500034, GIBCO, Hangzhou, China) for 1–2 mins at 37°C in a humidified atmosphere consisting of 5% CO2 separately, where NCI-H358 and HCC827 were both cultured in RPMI-1640 medium. The antibodies included N-cadherin (1:3000, ab98952, Abcam), E-cadherin (1:2000, 20874-1-AP, Proteintech), vimentin (1:2000, ab92547, Abcam), p-JAK (1:500, ab32101, Abcam), p-STAT3 (1:500, ab76315, Abcam), JAK (1:1000, 3230, CST), STAT3 (1:1000, 06-596, Merck), and GAPDH (1:5000, 70-Mab5465-040, MultiSciences).
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Taxus wallichiana var. chinensis (Pilg.) Florin was purchased from the pharmacy in the First Affiliated Hospital of Zhejiang Chinese Medical University (Hangzhou, China), which was manufactured by the Ningbo Taikang Yew Biological Engineering Company (Batch No. 100513). Human lung cancer (LC) cell lines, including A549, NCI-H358, HCC827, and MRC-5, were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). They were cultured by 0.25% trypsin digestion in the mediums of F-12K (21127-022, GIBCO, Hangzhou, China), RPMI-1640 (C11875500BT, GIBCO, Hangzhou, China), and MEM (41500034, GIBCO, Hangzhou, China) for 1–2 mins at 37°C in a humidified atmosphere consisting of 5% CO2 separately, where NCI-H358 and HCC827 were both cultured in RPMI-1640 medium. The antibodies included N-cadherin (1:3000, ab98952, Abcam), E-cadherin (1:2000, 20874-1-AP, Proteintech), vimentin (1:2000, ab92547, Abcam), p-JAK (1:500, ab32101, Abcam), p-STAT3 (1:500, ab76315, Abcam), JAK (1:1000, 3230, CST), STAT3 (1:1000, 06-596, Merck), and GAPDH (1:5000, 70-Mab5465-040, MultiSciences).
2
Comprehensive Protein Analysis Workflow
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Western blots were performed by separating proteins on SDS-PAGE gels followed by protein transfer to PVDF membranes (Bio-Rad). The antibodies used were: anti-PDGFD (sc-137030, Santa Cruz), anti-SOX2 (AF-2018, R&D), anti-BRACHYURY (sc-166962, Santa Cruz), anti-ERKs (4696, Cell Signaling Technology), anti-Phospho ERKs (4370, Cell Signaling Technology), anti-PDGFR-β (3169, Cell Signaling Technology), anti-Phospho PDGFR-β (Tyr1021) (2227, Cell Signaling Technology), anti-PECAM1 (222783, Abcom), anti-Phospho STAT3 (9145, Cell Signaling Technology), anti-STAT3 (9139, Cell Signaling Technology), anti-KDR (9698, Cell Signaling Technology), anti-HSP90 (7411, Cell Signaling Technology), anti-TUBULIN (RM2007, Ray Antibody), anti-β-ACTIN (RM2001, Ray Antibody), anti-GAPDH (70-Mab5465-040, MultiSciences), anti-HISTONE 3 (GB13102-1, Servicebio), Goat anti-mouse IgG (GAM0072, MultiSciences), Goat anti-rabbit IgG (GAR0072, MultiSciences), Rabbit anti-goat IgG (RAG0072, MultiSciences), anti-PDGFB (PA1-27394, Invitrogen), anti-uPA (17968-1-AP, Proteintech) and anti-PLASMIN (66399-1-Ig, Proteintech). The bands were visualized using a GBOX-CHEMI-XX8 device (SYNGENE).
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