ECV304 stably transfected with eNOS-GFP cells were grown overnight at 40% confluency on coverslips in 12-well plates. The cells were treated with control, sFRP4, BK and sFRP4 + BK respectively. Following treatment, cells were placed in live-cell chambers and eNOS localization was observed using an Olympus IX71 epifluorescence microscopy system equipped 209 with a DP71 camera.
Ix71 epifluorescence microscopy system
Manufactured by Olympus
The IX71 is an epifluorescence microscopy system designed for research applications. It provides optical and mechanical components to capture fluorescent images of samples. The system includes a motorized stage, filter cubes, and a camera interface to enable imaging of fluorescently labeled specimens.
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Lab products found in correlation
2 protocols using ix71 epifluorescence microscopy system
1
Visualizing eNOS Localization in ECV304 Cells
2
Chick Vascular Bed Hypoxia and Ischemia
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The chick vascular beds were treated with global hypoxia and ischemic for 2 h. Next, the isolated vascular beds from egg yolk were washed with 1X PBS. The vascular beds were fixed with 4% of paraformaldehyde and permeabilized with 0.1% of Triton X. Finally, they were stained with Alexa Fluor-phalloidin (10 µM) and incubated for 10 min. The vascular beds were then washed with 1X PBS. Images were captured at 20X magnification using Olympus IX71 epifluorescence microscopy system equipped with a DP71 camera.
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