Murashige and skoog ms media

Manufactured by Duchefa Biochemie

Sourced in Germany

Murashige and Skoog (MS) media is a type of plant cell culture medium developed by Toshio Murashige and Folke Skoog. It provides a balanced formulation of essential nutrients, vitamins, and growth regulators to support the growth and development of plant cells, tissues, and organs in a controlled laboratory environment.

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Lab products found in correlation

Azoxystrobin, by Merck Group (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) Milli q water, by Merck Group (1 mentions) Milli q system, by Merck Group (1 mentions) Sucrose, by Merck Group (1 mentions) Nex 5n camera, by Sony (1 mentions)

Close spelling variants of this product

Murashige and Skoog media Murashige and Skoog (MS) Murashige Skoog media Murashige and Skoog MS media

5 protocols using murashige and skoog ms media

Arabidopsis Seed Germination Assay

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(which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
Arabidopsis seeds were surface sterilized by consecutive treatments of 5 min 70% (v/v) ethanol with 0.05% (w/v) sodium dodecyl sulfate (SDS) and 5 min 95% (v/v) ethanol. Then seeds were sown on half-strength Murashige and Skoog (½ MS) media (Duchefa Biochemie) containing 1% agar, supplemented with 1 μM (-)-GR24 or with 0.01 % DMSO (control). Seeds were stratified at 4 °C (2 days in dark) then transferred to the growth chamber at 22 °C, under 20-30 µE /m 2 /sec of white light in long day conditions (16 hr light/ 8 hr dark). Seedlings were photographed and hypocotyl lengths were quantified using ImageJ (71) . 2 plates of 10-12 seeds were sown for each genotype x treatment. Using Student t-tests, no statistically significantly different means were detected between plates. The data from the 20-24 seedlings were then used for a one-way ANOVA.

Guercio A.M., Torabi S., Cornu D., Dalmais M., Bendahmane A., Signor C.L., Pillot J., Bris P.L., Steinmetz V., Rameau C., Gutjahr C., Germain A.d.S., & Shabek N. (2021). Structural and Functional Analyses Explain Pea KAI2 Receptor Diversity and Reveal Stereoselective Catalysis During Signal Perception.

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Quantifying Hypocotyl Length in Arabidopsis

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Arabidopsis seeds were surface sterilized by consecutive treatments of 5 min 70% (v/v) ethanol with 0.05% (w/v) sodium dodecyl sulfate (SDS) and 5 min 95% (v/v) ethanol. Then seeds were sown on half-strength Murashige and Skoog (½ MS) media (Duchefa Biochemie) containing 1% agar, supplemented with 1 μM (–)-GR24 or with 0.01 % DMSO (control). Seeds were stratified at 4 °C (2 days in dark) then transferred to the growth chamber at 22 °C, under 20-30 µE /m²/sec of white light in long day conditions (16 hr light/ 8 hr dark). Seedlings were photographed and hypocotyl lengths were quantified using ImageJ^{77 (link)}. 2 plates of 10-12 seeds were sown for each genotype x treatment. Using Student t tests, no statistically significantly different means were detected between plates. The data from the 20 to 24 seedlings were then used for a one-way ANOVA.

Guercio A.M., Torabi S., Cornu D., Dalmais M., Bendahmane A., Le Signor C., Pillot J.P., Le Bris P., Boyer F.D., Rameau C., Gutjahr C., de Saint Germain A, & Shabek N. (2022). Structural and functional analyses explain Pea KAI2 receptor diversity and reveal stereoselective catalysis during signal perception. Communications Biology, 5, 126.

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Seed Surface Sterilization for Tissue Culture

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Surface sterilization was performed by
washing the seeds placed in a cylinder with running tap water for
a minute. Then, they were immersed first in 70% ethyl alcohol (Adwic,
ARE) for 2 min and then in a 5% sodium hypochlorite solution for 5
min with continuous shaking. Finally, the seeds were rinsed with double-distilled
sterile water three times under sterile conditions. Sterilized seeds
were transferred aseptically to half-strength Murashige and Skoog
(MS) media (Duchefa, Germany) (2.2 g/L) supplemented with 30 g/L sucrose
(Adwic, ARE) and 6 g/L agar (Bioworld). They were incubated at 20
°C in a growth cabinet for a 16 h photoperiod. The percentage
of seed germination was calculated after 20 days of cultivation using
the following formula: (number of germinated seeds/total number of
cultured seeds) × 100.^{54 (link)}

Hamdan D.I., Fayed M.A, & Adel R. (2021). Echinops taeckholmiana Amin: Optimization of a Tissue Culture Protocol, Biological Evaluation, and Chemical Profiling Using GC and LC-MS. ACS Omega, 6(20), 13105-13115.

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Quantitative Analysis of Azoxystrobin

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Analytical grade azoxystrobin (purity 99.4%) and sucrose (≥99.5%) were purchased from Sigma-Aldrich (Copenhagen, Denmark). azoxystrobin free acid ((E)-2-(2-((6-(2-cyanophenoxy)pyrimidin-4-yl)oxy)phenyl)-3-methoxyacrylic acid) (10 ng/µL in Acetonitrile) was procured from Dr. Ehrenstorfer GmbH (Augsburg, Germany). Acetonitrile and methanol (TOF/MS grade) were obtained from Fisher Chemical (Roskilde, Denmark). Milli-Q water was generated from a Milli-Q system (Millipore, MA, USA). Murashige and Skoog (M&S) media was purchased from Duchefa Biochemie (Harlem, The Netherlands).

Dionisio G., Gautam M, & Fomsgaard I.S. (2019). Identification of Azoxystrobin Glutathione Conjugate Metabolites in Maize Roots by LC-MS. Molecules, 24(13), 2473.

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Drought Stress Response in Transgenic Rice

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Transgenic and NT control plants were germinated on Murashige and Skoog (MS) media (Duchefa, Haarlem, Netherlands) at 28 °C for 4 days, and eighteen seedlings of each transgenic lines and NT were transplanted into soil pots (4 × 4 × 6 cm; four plants per pot) and grown for 4 weeks in a greenhouse (16‐h light/8‐h dark cycle) at 28–30 °C. Each pot had the same size of holes in the bottom, and they were all placed in a single tray to synchronize watering. Drought stress was simultaneously applied to all the rice plants by first adding no water to the soil pots for 5 days and then re‐watering. Drought‐induced symptoms were monitored by imaging transgenic and NT plants at the indicated time points using a NEX‐5N camera (Sony, Tokyo, Japan).

Lee D., Chung P.J., Jeong J.S., Jang G., Bang S.W., Jung H., Kim Y.S., Ha S., Choi Y.D, & Kim J. (2017). The rice OsNAC6 transcription factor orchestrates multiple molecular mechanisms involving root structural adaptions and nicotianamine biosynthesis for drought tolerance. Plant Biotechnology Journal, 15(6), 754-764.

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