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2 protocols using c18 0methyl ester

1

Mass Spectrometry Reagent Standards

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Purified water was obtained using the Milli-Qplus185 system (Millipore, Billerica, MA, USA). LC–MS grade methanol (MeOH), acetonitrile (ACN), formic acid and LC grade ethanol (EtOH), standard mix for GC–MS, containing grain fatty acid methyl ester mixture (C8-C28), 2-propanol and analytical grade heptane were purchased from Sigma-Aldrich ChemieGmbH (Steinheim, Germany). C18:0methyl ester and N,O-bis(trimethylsilyl) trifluoroacetamide with 1% trimethylchlorosilane were obtained from Pierce Chemical Co (Rockford, IL, USA). Silylation grade pyridine was purchased from VWR International BHDProlabo (Madrid, Spain). The API-TOF reference mass solution kit (G1969-850001) and tuning solutions, ESI-L low concentration tuning mix (G1969-85000) and ESI-TOF Biopolymer Analysis referencemasses (G1969-850003) were purchased from Agilent Technologies (Santa Clara, CA, USA).
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2

GC-MS Standard Mix Preparation

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Standard mix for GC-MS, containing grain fatty acid methyl ester mixture (C8:0-C22:1n9), and LC-MS grade organic solvents, acetonitrile, 2-propanol and analytical grade heptane were from Fluka Analytical (Sigma-AldrichChemie GmbH, Steinheim, Germany). C18:0 methyl ester, N,O-bis(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane were from (Pierce Chemical Co, Rockford, IL, USA). Silylation grade pyridine was from VWR International BHD Prolabo (Madrid, Spain).
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