Glacial acetic acid

Phosphate-buffered saline (PBS) (0.1 M, pH 7.2) was bought from Beijing Oka Biological Technology Co., Ltd. (Beijing, China). Glacial acetic acid and absolute ethanol were purchased from Guangzhou Chemical Reagent Factory (Guangzhou, China).

Acetonitrile (HPLC grade) was obtained from Fisher Corporation (Waltham, MA, USA). Adenosine-5-two sodium phosphate (C₁₀H₁₃N₅Na₂O₁₀P₂) was purchased from Sigma-Aldrich Co (St. Louis, MO, USA). Sodium ferulic acid was obtained from Shanghai Yuan Ye Biotechnology Co., Ltd. (Shanghai, China). Glacial acetic acid, dimethyl sulfoxide (DMSO), and three sodium citrate (two water) C₆H₅Na₃O₇·2(H₂O) were of analytical grade and acquired from Guangzhou Chemical Reagent Factory (Guangzhou, China). Sodium chloride injection (0.9%, W/V) was purchased from Sichuan Cologne Pharmaceutical Co., Ltd. (Chengdu, China). CR decoction pieces (Product Batch No. 180802731) were manufactured by Kangmei Pharmaceutical Co., Ltd. (Guangdong, China) and deposited in the traditional Chinese Medicine Laboratory of Puning Production Base of Kangmei Pharmaceutical. Standards of vanillin, ferulic acid, senkyunolide I, senkyunolide H, coniferyl ferulate, Z-ligustilide, butylphthalide, senkyunolide A, and levistilide A (purities ≥ 98% by HPLC) were purchased from Chengdu Pufei De Biotech Co., Ltd. (Chengdu, China). Japanese big-eared white rabbits, male, weighing about 2.5 kg, were provided by Chengdu Dashuo Biotechnology Co., Ltd. (Chengdu, China).

Alpha‐lipoic acid (D118666), 8‐aminooctanoic acid (an internal standard for ALA, A165345) were obtained from Aladdin (shanghai, china). The methanol and acetonitrile (ACN) of LC‐ESI‐MS/MS grade were purchased from Sigma‐Aldrich (St. Louis, USA). Chloroform, glacial acetic acid, and ammonia of AR grade were obtained from Guangzhou Chemical Reagent Factory (Guangzhou, China).