Tc 1 cells

Manufactured by American Type Culture Collection

Sourced in United States

TC-1 cells are a murine cell line derived from primary lung epithelial cells transformed with the human papillomavirus (HPV) type 16 E6 and E7 oncogenes. These cells are commonly used in research for the study of HPV-associated cancers.

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Lab products found in correlation

Penicillin, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Mda mb 231, by American Type Culture Collection (2 mentions) Female c57bl 6 mice, by Charles River Laboratories (2 mentions) Tc1 cells, by Thermo Fisher Scientific (1 mentions) Crl 5800, by American Type Culture Collection (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Wild type c57bl 6 mice, by Charles River Laboratories (1 mentions)

5 protocols using tc 1 cells

Effects of Sleep Deprivation on Lung Cancer

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TC1 cells (ATCC, Manassas, VA, USA) derived from primary lung epithelial cell tumors of C57/B6 mice were cultured at 37°C, 95% air, 5% CO₂ incubator in full tumor medium as recommended by the American Type Culture Collection (ATCC). TC1 cells were cultured in RPMI-1640 medium with 2 mmol/L L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 10 mmol/L HEPES, and 1.0 mmol/L sodium pyruvate supplemented with 2 mmol/L nonessential amino acids, penicillin and streptomycin, and 10% FBS (Life Technologies, Foster City, CA).
Mice exposed to either control sleep conditions (SC; n = 12) or SF (n = 12) were inoculated with TC1 cells (1 × 10⁵ cells in 0.2 mL PBS) by subcutaneous injection into the right lower flank. Tumors volumes were estimated every 3 days by externally measuring its length and width with an electronic caliper. After 28 days from tumor injection during which the sleep-related exposures were continued, mice were sacrificed, blood collected, and tumors excised and weighed.
The human adenocarcinoma, non-small cell lung cancer cell line was obtained from ATCC (CRL-5800, ATCC, Manassas, VA) and used for experiments with OSA patients-derived plasma exosomes (see below).

Khalyfa A., Almendros I., Gileles-Hillel A., Akbarpour M., Trzepizur W., Mokhlesi B., Huang L., Andrade J., Farré R, & Gozal D. (2016). Circulating exosomes potentiate tumor malignant properties in a mouse model of chronic sleep fragmentation. Oncotarget, 7(34), 54676-54690.

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Culturing and Inoculating Mouse Lung Cancer Cell Lines

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We used TC-1 cells (ATCC CRL- 2785) and 3LLC (ATCC CRL-1642) in all experiments. Both cell lines are derived from primary lung epithelial cells of C57/B6 mice and were cultured at 37°C, 95% air, 5% CO2 incubator in full tumor medium as recommended by the American Type Culture Collection (ATCC, Rockville, MD, USA). TC-1 cells were cultured in RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 2 mM non-essential amino acids, penicillin and streptomycin and 10% fetal bovine serum, all supplied by Gibco, Life Technologies (Grand Island, NY, USA), and selected. with G418 (Gibco, Life Technologies, Grand Island, NY, USA) and Hygromycin B (Calbiochem, EMD Millipore Corporation, Billerica, MA, USA). 3LL cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), L-glutamine, penicillin and streptomycin, all supplied from (Gibco, Life Technologies, Grand Island, NY, USA). Study mice were inoculated with TC-1 or 3LL cells [1×10⁵ cells in 0·2 ml phosphate-buffered saline (PBS)] by subcutaneous injection into the right lower flank or right thigh for selected experiments.

Hakim F., Wang Y., Zhang S.X., Zheng J., Yolcu E.S., Carreras A., Khlayfa A., Shirwan H., Almendros I, & Gozal D. (2014). Fragmented sleep accelerates tumor growth and progression through recruitment of tumor-associated macrophages and TLR4 signaling. Cancer research, 74(5), 1329-1337.

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TC-1 Cells With Oncogenes and Luciferase

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TC-1 cells expressing the oncogenes HaRas, HPV16-E6 and HPV16-E719 (link) were obtained from the American Type Culture Collection (LGC Promochem, Molsheim, France). Its variant TC-1 P3 (A15) cell line with downregulated MHC class I expression20 (link) was kindly provided by Dr TC Wu (MD, USA). TC-1 cells expressing luciferase (LC-1 Luc) were then generated by lentiviral infection.18 (link) THP-1 human monocyte-derived cells were obtained from The American Type Culture Collection, Manassas, Virginia, USA). Cells were grown in complete medium (CM) consisting of RPMI-1640 supplemented with 10% fetal calf serum, 100 U/mL penicillin, 100 µg/mL streptomycin, 2 mM L-glutamine and 50 µM 2-mercaptoethanol.
Specific pathogen‐free, female C57BL/6 wild-type mice aged 7–10 weeks (Charles River) were used in compliance with the ethical directives of the Swiss and Spanish veterinary authorities. They were housed in appropriated animal care facilities during the experimental period and handled following the international guidelines required for experimentation with animals.

Arribillaga L., Echeverria I., Belsue V., Gomez T., Lozano T., Casares N., Villanueva L., Domingos-Pereira S., Romero P.J., Nardelli-Haefliger D., Hervás-Stubbs S., Sarobe P., Rodriguez M.J., Carrascosa J.L., Zürcher T, & Lasarte J.J. (2020). Bivalent therapeutic vaccine against HPV16/18 genotypes consisting of a fusion protein between the extra domain A from human fibronectin and HPV16/18 E7 viral antigens. Journal for Immunotherapy of Cancer, 8(1), e000704.

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Murine and Human Cancer Cell Culture

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TC-1 cells (murine lung cancer
cell line) were from the American Type Culture Collection (ATCC, Rockville,
MD, USA) and grown in RPMI 1640, supplemented with 10% fetal bovine
serum (FBS) and 1% of 100 μg/mL streptomycin and 100 IU/mL penicillin
(1% P/S). M-Wnt cells (murine mammary gland cell lines) were from
Dr. Stephen D. Hursting’s lab at The University of Texas at
Austin. M-Wnt cells were grown in a similar medium as TC-1, with an
additional supplement of 1% Glutamax. Human breast adenocarcinoma
cells (MDA-MB-231) were from ATCC and grown in DMEM supplemented with
5% FBS and 1% P/S. All cell culture reagents were from Invitrogen
(Life Technologies, Carlsbad, CA, USA). Female C57BL/6 mice (6–8
weeks old) were from Charles River Laboratories (Wilmington, MA, USA).

Naguib Y.W., Rodriguez B.L., Li X., Hursting S.D., Williams RO I.I.I, & Cui Z. (2014). Solid Lipid Nanoparticle Formulations of Docetaxel Prepared with High Melting Point Triglycerides: In Vitro and in Vivo Evaluation. Molecular Pharmaceutics, 11(4), 1239-1249.

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Murine and Human Cancer Cell Culture

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TC-1 cells (murine lung cancer cell line) were from the American Type Culture Collection (ATCC, Rockville, MD, USA) and grown in RPMI 1640, supplemented with 10% fetal bovine serum (FBS) and 1% of 100 μg/ml streptomycin and 100 IU/ml penicillin (1% P/S). M-Wnt cells (murine mammary gland cell lines) were from Dr. Stephen D. Hursting’s lab at The University of Texas at Austin. M-Wnt cells were grown in a similar medium as TC-1, with an additional supplement of 1% Glutamax^®. Human breast adenocarcinoma cells (MDA-MB-231) were from ATCC and grown in DMEM supplemented with 5% FBS and 1% P/S. All cell culture reagents were from Invitrogen^™ (Life Technologies, Carlsbad, CA, USA). Female C57BL/6 mice (6–8 weeks old) were from Charles River Laboratories (Wilmington, MA, USA).

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