Su dhl 1 cell line

The NCI-H3122 cell line harboring the fusion gene EML4-ALK variant 1, and the Karpas 299 cell line and the SU-DHL-1 cell line harboring the fusion gene NPM-ALK were purchased from American Type Culture Collection (Manassas, VA) and cultured in RPMI1640 medium (Gibco, Grand Island, NY) containing 10% fetal bovine serum (Gibco). Cells were maintained in a cell culture incubator at 37°C in a humidified atmosphere with 5% CO₂.
Cell viability was evaluated by a WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] assay (Dojindo Molecular Technologies, Inc., Rockville, MD). Cells were plated in 96-well plates and cultured overnight to allow cells to attach, and then the drug was added at indicated concentrations for 96 hours. Cell culture media containing the drug were washed, 10% WST-8 dye (100 μl) was added to each well and incubated for an additional hour, and the absorbance at 450 nm was measured in a microplate reader (Molecular Devices, Sunnyvale, CA). Cell growth inhibition was evaluated as the ratio of the absorbance of the drug-treated samples to that of the DMSO-treated control and analyzed by Prism 6 software. All experiments were carried out in triplicate.