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2 protocols using p dimethyl amino benzaldehyde

1

Extracellular Matrix Protein Analysis

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Glycine, phenazine methosulfate fluoride (PMSF), Bradford reagent, and Tris-HCl were purchased from Sigma (Sigma Chemical Co., USA). Carbopol 934, propylene glycol, gentamicin sulfate, l-hydroxyproline, and p-dimethyl-amino-benzaldehyde were obtained from Himedia (Himedia Pvt. Labs., India). bFGF, COL3A1, Smad-2, Smad-3, Smad-4, and Smad-7, and β-actin primary and secondary antibodies and BCIP- (5-bromo-4-chloro-3′-indolyphosphate p-toluidine salt-) NBT reagents were procured from Santa Cruz (Santa Cruz, USA). PVDF membrane was from Millipore (Millipore Corp., USA) and Western Max-HRP-Chromogenic detection kit was purchased from Amresco (Amresco, USA). All other chemicals and reagents not mentioned were of the analytical grade.
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2

Mycobacterial Growth Assay Protocol

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All Media chemicals Middlebrook 7H9 broth, Middlebrook 7H10 agar, albumin/dextrose/catalase (ADC), oleic acid/albumin/dextrose/catalase (OADC) supplements were purchased from BD Biosciences (USA). Tween-80, Roswell Park Memorial Institute Medium (RPMI), Phorbol 12-myristate 13-acetate (PMA), Amikacin, Fetal bovine serum (FBS), Isoniazid (INH), Rifampicin (RIF), Streptomycin (STP) and DCFDA (dichlorodihydro-fluorescein diacetate) were obtained from Sigma Chemical Co. (St.Louis, MO, USA). Chloroform, hexane (C6H14), diethyl ether ((C2H5)2O), sulfuric acid (H2SO4), and iodine (I), Methanol (CH3OH), Triton X-100, crystal violet (CV), peptone, p-dimethyl aminobenzaldehyde, ethambutol (EMB) were purchased from Himedia (Mumbai, India). Dimethyl sulfoxide (DMSO), Potassium chloride (KCl), Sodium chloride (NaCl), di-Sodium Hydrogen Orthophosphate (Na2HPO4), Potassium Di-hydrogen Orthophosphate (KH2PO4), Glycerol, magnesium chloride (MgCl2), calcium chloride (CaCl2), were obtained from Fischer Scientific. Agarose was purchased from CDH India.
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