Cp 3800 gc system

GC-IT-MS analysis was performed using a Varian CP-3800 GC system equipped with a Varian CP-8400 Autosampler and a Varian 4000 ion trap EI MS detector system (Varian, Palo Alto, CA, USA). 1 µl of each reactant was injected into the GC-IT-MS (with a split ratio of 25∶1) through a VF-1MS capillary column (30 m length×0.25 mm i.d., 0.25 µm film thickness) with helium at a constant flow of 1.0 ml min⁻¹ as the carrier gas. The oven temperature was held at 100°C for 2 min, then ramped to 300°C at a rate of 10°C min⁻¹ and held for 10 min. The mass data collected in the electron ionization mode with the ionization energy of 70 eV were used for full scan at m/z 50–1,000.

The chromatographic method proposed by Dugheri et al. was used [60 (link)], with a Varian CP3800 GC system coupled to a Varian Saturn 2200 Ion-Trap as the detector (scan mode, 45–300 m/z, EI energy 70 eV).
The column was a DB 35-MS-UI (Agilent J&W), and the 1079 injector port (SCION, Instruments, Amundsenweg, The Netherlands) was provided with a 0.75 mm internal diameter liner. The oven settings were isotherm of 50 °C for 1 min, followed by a linear temperature ramp of 10 °C min⁻¹ to 260 °C. Helium was used as the carrier gas, set at a flow rate of 1.2 mL min⁻¹. The absolute quantity of PFB-FA-oxime was calculated on a regression curve obtained via automatic direct injection (1 μL) of hexane solutions in the GC system (0, 5, 10, 20, and 40 mg L⁻¹ of PFB-FA-oxime, respectively) to assess the recovery of the method. When operating in cooling SPME mode, the SDLE module, installed on the PAL 6-position agitator, was set at 10 °C using Chronos software.

The six final wines obtained using industrial conditions were analyzed for major volatile compounds by gas chromatographic–flame ionization detection (GC-FID) by an external analytical service (L.A.A.E., Zaragoza, Spain) according to Ortega et al. (2001) (link). In summary, 3 mL of each wine were diluted with 7 mL of water, salted with 4.5 g of ammonium sulfate and extracted with 0.2 mL of dichloromethane. The extract was injected in the split mode into a Varian CP-3800 GC system (Palo Alto, CA, United States), separated on a DB-WAX polyethylene glycol column (30 m × 0.32 mm and 0.5 μm film thickness) from J&W Scientific (Folsom, CA, United States), and detected by FID.