Bismuth oxide (Bi2O3, 99.99%), 4-nitro-o-phenylenediamine (≥99%), boric anhydride (≥98%), boron standard solution (999.5 mg L−1 ± 20 mg L−1), bismuth standard for ICP traceCert® (1000 mg L−1 Bi in nitric acid), potassium hydroxide (KOH, ≥85%, pellets), Nafion (≤100%), lithium iodide (99.9%), 4-tert-butylpyridine (98%), guanidinium thiocyanate (99%), 1-methyl-3-propylimidazolium iodide (99.99%), acetonitrile (≥99.9%), poly(vinyl acetate) (99.9%), absolute ethanol (99.5%), eosin B (97%) and indium tin oxide (ITO) coated glass slides (15 Ω, 30 × 30 × 0.7 mm) were purchased from Sigma-Aldrich, South Africa.
Eosin b
Manufactured by Merck Group
Sourced in United States, Germany
Eosin B is a synthetic dye commonly used as a biological stain in laboratory settings. It is a red, crystalline powder that is soluble in water and alcohol. Eosin B is commonly used to stain cellular structures for microscopic analysis, particularly in the fields of histology and cytology.
Automatically generated - may contain errors
Lab products found in correlation
Porcine liver esterase, by Merck Group (2 mentions)
2 ethyl 2 oxazoline etox, by Merck Group (2 mentions)
Methyl tosylate meots, by Merck Group (2 mentions)
Lucirintpo l ethyl 2 4 6 trimethylbenzoylphenylphosphinate, by ABCR (2 mentions)
Deuterated chloroform, by Merck Group (2 mentions)
2 2 ethylenedioxy diethanethiol deg dichloromethane, by Merck Group (2 mentions)
Guanidinium thiocyanate, by Merck Group (1 mentions)
Absolute ethanol, by Merck Group (1 mentions)
Polyvinyl acetate, by Merck Group (1 mentions)
Bismuth oxide, by Merck Group (1 mentions)
1 methyl 3 propylimidazolium iodide, by Merck Group (1 mentions)
4 nitro o phenylenediamine, by Merck Group (1 mentions)
Indium tin oxide coated glass slides, by Merck Group (1 mentions)
Potassium hydroxide, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Lithium iodide, by Merck Group (1 mentions)
4 tert butylpyridine, by Merck Group (1 mentions)
Nafion, by Merck Group (1 mentions)
Acridine orange, by Merck Group (1 mentions)
Dab substrate kit, by Vector Laboratories (1 mentions)
12 protocols using eosin b
1
Synthesis of Lead-Free Perovskite Solar Cells
2
Embedding Specimens in Methacrylate Resin
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Fixed specimens were dehydrated, infiltrated and embedded in eosin dyed methacrylate resin. For dehydration, the specimens were transferred into an ascending methanol series (10% increments until 90%, then 95% and 100%, 1 h each, constant gentle rocking). Following dehydration, the specimens were infiltrated with JB-4 infiltration solution (Polysciences, Inc., Warrington, PA, USA) for 72 h and embedded in JB-4 embedding solution. Eosin B (Sigma-Aldrich, St. Louis, MO, USA) (0.275 g/100 mL) and acridine orange (Sigma-Aldrich, St. Louis, MO, USA) (0.055 g/100 mL) were added to both solutions [9 (link)].
For embedding, the specimens were transferred from the infiltration solution into embedding molds filled with embedding solution. Embryos were placed, head down and carefully positioned with their cranio-caudal axis perpendicular to the future block surface as soon as the viscosity of the embedding solution started to increase. For this, forceps or a blunt needle were used. As soon as the embryos were fixed by the hardening resin, block holders were placed on the embedding molds. Then, the molds were fully filled and sealed air proof using mineral oil to prevent oxygen to affect polymerization. After polymerization at room temperature for 12–24 h, the resin blocks were baked at 90 °C for 24–48 h to speed up the hardening process [9 (link),23 (link)].
For embedding, the specimens were transferred from the infiltration solution into embedding molds filled with embedding solution. Embryos were placed, head down and carefully positioned with their cranio-caudal axis perpendicular to the future block surface as soon as the viscosity of the embedding solution started to increase. For this, forceps or a blunt needle were used. As soon as the embryos were fixed by the hardening resin, block holders were placed on the embedding molds. Then, the molds were fully filled and sealed air proof using mineral oil to prevent oxygen to affect polymerization. After polymerization at room temperature for 12–24 h, the resin blocks were baked at 90 °C for 24–48 h to speed up the hardening process [9 (link),23 (link)].
3
Immunohistochemical Analysis of SET/TAF-Iβ in Colon Cancer
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Colon carcinoma tissue array slides (CO243a) including colon carcinoma tissues and matched adjacent normal colon tissues were purchased from US Biomax (Derwood, MD, USA). These slides contain formalin-fixed, paraffin-embedded normal colon tissues and colon cancer tissues. First, slides were deparaffinized in xylene and rehydrated in graded ethanol. Antigens were retrieved by heating with microwave oven in 0.01 M citrate buffer (pH 6.0). Endogenous peroxidase activity was quenched by incubating in 3% hydrogen peroxide solution for 6 min. Next, slides were incubated with normal goat serum for 30 min at room temperature for blocking. Subsequently, the slides were incubated with primary antibody against SET/TAF-Iβ (1:500, ab1183 from Abcam) for 2 h at room temperature followed by incubation with biotinylated anti-rabbit secondary antibody and streptavidin-horseradish peroxidase (Zymed Laboratories, South San Francisco, CA, USA). DAB substrate kit (SK-4100, Vector Laboratories, Newark, CA, USA) was used as a chromogen and Eosin B (Sigma-Aldrich) was used for counterstaining.
4
Synthesis and Characterization of Degradable Oxazoline Polymers
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2-Ethyl-2-oxazoline (EtOx) and methyl tosylate (MeOTs) were purchased
from Sigma-Aldrich (Vienna, Austria), purified by distillation over sodium
sulfate and stored in inert atmosphere. 2,2′-(Ethylenedioxy)diethanethiol
(DEG), dichloromethane, ethanol and deuterated chloroform were acquired from
Sigma-Aldrich (Vienna, Austria) and used without purification. Glycol
dimercaptoacetate (GDMA) was kindly provided by Bruno Brock (Marschacht,
Germany) and used without any further purification. The photoinitiator Lucirin
TPO-L®, ethyl-2,4,6-trimethylbenzoylphenylphosphinate, was
purchased from abcr GmbH (Karlsruhe, Germany). Deionized water was produced
through reverse osmosis. 2-But-3′-enyl-2-oxazoline (Bu⁼Ox),
2-nonyl-2-oxazoline (NonOx) and 2-dec-9′-enyl-2-oxazoline (Dc⁼Ox)
were synthesized according to literature protocols [22 –24 (link)],
purified by distillation and flash chromatography using chloroform as an eluent.
For the degradation studies, the enzymes (namely porcine liver esterase and
rabbit liver esterase), Eosin B, and the buffer solutions (pH = 4, 6, 8, and 10)
were acquired from Sigma-Aldrich (Vienna, Austria) and used as received.
from Sigma-Aldrich (Vienna, Austria), purified by distillation over sodium
sulfate and stored in inert atmosphere. 2,2′-(Ethylenedioxy)diethanethiol
(DEG), dichloromethane, ethanol and deuterated chloroform were acquired from
Sigma-Aldrich (Vienna, Austria) and used without purification. Glycol
dimercaptoacetate (GDMA) was kindly provided by Bruno Brock (Marschacht,
Germany) and used without any further purification. The photoinitiator Lucirin
TPO-L®, ethyl-2,4,6-trimethylbenzoylphenylphosphinate, was
purchased from abcr GmbH (Karlsruhe, Germany). Deionized water was produced
through reverse osmosis. 2-But-3′-enyl-2-oxazoline (Bu⁼Ox),
2-nonyl-2-oxazoline (NonOx) and 2-dec-9′-enyl-2-oxazoline (Dc⁼Ox)
were synthesized according to literature protocols [22 –24 (link)],
purified by distillation and flash chromatography using chloroform as an eluent.
For the degradation studies, the enzymes (namely porcine liver esterase and
rabbit liver esterase), Eosin B, and the buffer solutions (pH = 4, 6, 8, and 10)
were acquired from Sigma-Aldrich (Vienna, Austria) and used as received.
5
Synthesis and Characterization of Degradable Oxazoline Polymers
6
Intracellular Ca2+ Storage Inhibition
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Several inhibitors were dissolved in test solution, and plant leaves were pretreated for 30 min. After three to five washes, the leaves were used for experiments. In this study, 200 μM ruthenium red (Sigma-Aldrich, St. Louis, MO, USA) was used as an inhibitor of intracellular Ca2+ storage, 10 μM eosin B (Sigma-Aldrich) was used as an inhibitor of the plasma membrane calcium pump Ca2+-ATPase, and 50 μM diphenyleneiodonium chloride (Sigma-Aldrich) was used as an inhibitor of NADPH oxidase.
7
Sectional Imaging and 3D Reconstruction of Outflow Tract Development
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The 7 μm-thick serial sections from E14.5 control, Bcar1SM22KO, and Bcar1PAX3KO embryos were stained with H&E and scanned with the NanoZoomer HT slide scanner (Hamamatsu Photonics Ltd.). Image files were uploaded to the Amira software where they were aligned and processed to produce animations illustrating septation of the entire OFT. For High Resolution Episcopic Microscopy (HREM) analysis, embryos were fixed in 4% PFA overnight, dehydrated through an acetone series (50%, 80%, 100%, 100%, and 100%), then infiltrated with Technovit 8100 resin (Taab Laboratories) through a series of 50:50 (Technovit 8100: Acetone) for 24 h, 75:25 (Technovit 8100: acetone) for 24 h, and 100% Technovit 8100 for a final 24 h. All solutions had the addition of 1 mg/ml Eosin B (Sigma) and were performed at 4°C with constant agitation. Finally, embryos were embedded in Technovit 8100 under vacuum at 4°C. After 48 h, samples were imaged using the Optical HREM system (Indigo Scientific) using 405/495 nm Ex/Em filter set, at appropriate resolution to ensure the entire embryo was in the field of view, and using a slice thickness to provide approximately isotropic resolution. Image segmentation was performed by manual annotation. Both segmentation and volume rendering were performed with Amira and ImageJ software.
8
Effect of Lecithin on Crosslinked Hydrogels
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Differently crosslinked hydrogel matrices and the effect of lecithin on their properties were studied. Three different crosslinking mechanisms were studied: physical, ionic, and chemical. Agarose E was purchased from Condalab (Madrid, Spain), calcium chloride from Lach-Ner (Neratovice, Czech Republic), and sodium alginate, poly(vinyl alcohol), chitosan, epichlorohydrin, and L-α-Phosphatidylcholine (lecithin) were all purchased from Sigma-Aldrich (Prague, Czech Republic). Dyes used for diffusion experiments differed in charge: positively charged rhodamine 6G (Sigma-Aldrich, Prague, Czech Republic), negatively charged amido black 10B (Merck, Darmstadt, Germany), and eosin B (Sigma-Aldrich, Prague, Czech Republic) were used.
9
Histological Staining of Xenografts
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Formalin-fixed and paraffin-embedded xenografts were subjected to hematoxylin staining according to Mayer (Sigma-Aldrich, Steinheim, Germany), followed by an 0.1% eosin B staining (Sigma-Aldrich, Steinheim, Germany). After washing in increasing concentrations of ethanol (50%, 70% and 96% (w/v)) and xylol, slides were covered with Pertex® cover solution (PER 20000, medite GmbH, Burgdorf, Germany).
10
Phenolic Coumarin Compound Evaluation
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The phenolic coumarin ESC (Figure 9 ) was purchased from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA) and resuspended in dimethyl sulfoxide. Horse serum (HS), glutamine, penicillin, streptomycin, G-418, hygromycin B, eosin B, 2′,7′-dichlorodihydrofluorescein diacetate (H2DCF-DA), monochlorobimane (MCB), leupeptin, PMSF, protease/phosphatase inhibitors cocktail and anti-β-Actin antibody were purchased from Sigma-Aldrich (Sigma-Aldrich). Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal bovine serum (FBS) were purchased from Euroclone (Euroclone, Milan, Italy). TH antibody was purchased from Santa Cruz (Santa Cruz Biotecnology, Dallas, TX, USA). All chemicals used were of high purity analytical grade.
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