All data were acquired at Durham University Neuroimaging Centre using a 3T Magnetom Trio MRI system (Siemens Medical Systems, Erlangen, Germany) with standard gradients and a 32-channel head coil. T2*-weighted axial echo planar imaging (EPI) scans were acquired parallel to the anterior/posterior commissure line with the following parameters: field of view (FOV) = 212 × 212 mm, flip angle (FA) = 90°, repetition time (TR) = 2160 ms, echo time (TE) = 30 ms, number of slices (NS) = 35, slice thickness (ST) = 3.0 mm, interslice gap = 0.3 mm, matrix size (MS) = 64 × 64. Images for each task were collected as separate runs (280 volumes each per run). For each participant, an anatomical scan was acquired using a high-resolution T1-weighted 3D-sequence (NS: 192; ST: 1 mm; MS: 512 × 512; FOV: 256 × 256 mm; TE: 2.52 ms; TR: 2250 ms; FA 9°).
3t magnetom trio mri system
Manufactured by Siemens
Sourced in Germany
The 3T Magnetom Trio MRI system is a magnetic resonance imaging (MRI) device produced by Siemens. It operates at a field strength of 3 Tesla, providing high-resolution imaging capabilities for medical diagnosis and research applications. The system is designed to capture detailed images of the human body.
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10 protocols using 3t magnetom trio mri system
1
3T fMRI Acquisition Protocol for Neuroimaging
2
T2 MRI of Agarose-Embedded HADMSCs
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HADMSCs (5 × 106/group) were washed with PBS to remove free FAC, trypsinized, harvested, and uniformly embedded into 600 μL of 1% agarose to prepare cell phantoms in 600-μL Eppendorf tubes for in vitro MRI. T2-weighted MRI was performed on a 3T MAGNETOM Trio MRI system (Siemens, Germany), and the T2 values were determined using a multi-echo spin echo T2-weighted sequence with the following parameters: TR, 4000 ms; eight echo settings of 13, 26, 39, 52, 65, 78, 91, and 104 ms; matrix, 64 × 128; field of view, 50 × 100 mm; and a horizontal slice with 2-mm slice thickness at the center of each Eppendorf tube.
3
3T fMRI Acquisition Protocol
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Scanning was performed on a Siemens 3 T Magnetom TRIO MRI system with a 32-channel head coil in the National Magnetic Resonance Imaging Center at Bilkent University. Functional images were acquired over six runs in each session using a gradient echo planar imaging (EPI) sequence (TR = 2000 ms, TE = 30 ms, flip angle = 90°, FOV = 192 mm, 34 interleaved axial slices, voxel size = 3 mm × 3 mm × 3 mm with 0.3 mm interslice gap). After each session, high-resolution T1-weighed (MP-RAGE) anatomical images were acquired.
4
Neonatal Brain MRI Scoring System
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Brain MRI was performed using the Siemens 3T Magnetom Trio MRI system (Siemens, Erlangen, Germany) during natural sleep, on the same day as neurobehavioural assessments. The details of the imaging protocol have previously been published, and the T1-weighted and T2-weighted structural brain images were used for the current study.20 (link)
A validated neonatal brain MRI scoring system was used to assess brain maturation, injury and size, using two-dimensional brain metrics29 (link) and conventional methods of assessing brain injury.30 (link) Four regional abnormality scores were calculated based on assessment of injury, growth and maturation: cerebral white matter, cortical grey matter, deep grey matter (basal ganglia and thalamus) and cerebellar abnormality.31 A global abnormality score was computed using the sum of the four regional abnormality scores; higher scores indicate greater abnormality. Brain MRI were scored independently by one of four experienced neuroradiologists and neonatologists who had received training in this scoring system with excellent inter-rater and intrarater reliability.20 (link)
A validated neonatal brain MRI scoring system was used to assess brain maturation, injury and size, using two-dimensional brain metrics29 (link) and conventional methods of assessing brain injury.30 (link) Four regional abnormality scores were calculated based on assessment of injury, growth and maturation: cerebral white matter, cortical grey matter, deep grey matter (basal ganglia and thalamus) and cerebellar abnormality.31 A global abnormality score was computed using the sum of the four regional abnormality scores; higher scores indicate greater abnormality. Brain MRI were scored independently by one of four experienced neuroradiologists and neonatologists who had received training in this scoring system with excellent inter-rater and intrarater reliability.20 (link)
5
High-Resolution 3D T1-Weighted Brain MRI
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MRI was performed at 2 of the study sites, each using a Siemens 3T MAGNETOM Trio MRI system (Siemens, Erlangen, Germany), a 12-channel receive-only head coil, and the same acquisition protocol. For this study, three-dimensional T1-weighted Magnetization Prepared Rapid Gradient Echo datasets were obtained using the following measurements: non-isotropic voxels 0.7 × 0.7 × 1.2 mm, field of view 230 mm, repetition time 1800 ms, echo time 2.67 ms, and flip angle 9°.
6
Evaluating SAON Establishment via MRI
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Magnetic resonance imaging (MRI) (Magnetom Trio 3T MRI System; Siemens, Germany) with a phased-array body coil was performed to determine successful establishment of SAON 12 weeks after SAON induction, and also to confirm the correct position of the bone tunnel and placement of the cylindrical scaffold. For facilitating in vivo imaging examination, a specific posture device was used to obtain a highly reproducible emu position during MRI scanning (Supplementary Fig. S2 ). Coronal turbo spin-echo T2-weighted fat-saturated images (4000 ms repetition time; 96 ms echo time) were obtained with a slice thickness of 3 mm, an interslice distance of 0.3 mm providing a field of view of 300 mm × 300 mm and a matrix of 320 × 320 pixels.
7
Pseudo-continuous ASL MRI for Cerebral Blood Flow
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A Siemens Magnetom Trio 3T MRI system (Siemens Medical Systems; Erlangen, Germany) was used to acquire pseudo-continuous ASL [PCASL, (32 (link), 33 (link))] images for CBF recording and T1-weighted anatomical images. The PCASL was a gradient-echo echo-planar sequence, acquiring images with and without labeling in an interleaved fashion. The 14 slices (voxel size = 3.4 × 3.4 × 6 mm3; gap = 1.5 mm) were collected in ascending order from inferior to superior. A post-label delay of 1,250 ms between the end of the labeling pulse (label time = 1,600 ms) and image acquisition (slice-acquisition time = 45 ms; FOV = 220 mm2; matrix = 64 × 64; TR/TE = 4,000/18 ms) was introduced. A total of 40 pairs label and control images were acquired, resulting in a total acquisition time of 5 min 20 s. Two identical PCASL acquisition runs were conducted, one before and one after the threat-of-shock paradigm. Anatomical images were acquired applying a magnetization prepared rapid gradient echo (MPRAGE) sequence (inversion time = 1000 ms; voxel size = 1 × 1 × 1 mm3; TR/TE = 2000/3.4 ms).
8
Resting-state fMRI in Healthy Volunteers
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We acquired rsfMRI in 51 healthy, right-handed volunteers (mean age: 23.6 ± 5.9, range: 18–47, 32 men, 63%). None of the subjects reported a history of drug abuse, neurological or psychiatric disorder. The experimental protocol was reviewed and approved by Research Ethics Board of MNI. All subjects gave informed consent. Scans were acquired using a Siemens MAGNETOM Trio 3T MRI system at the MNI. High-resolution, T1-weighted, three-dimensional volume acquisition for anatomic localization (1-mm3 voxel size) and resting-state echoplanar T2*-weighted images with blood oxygenation level-dependent (BOLD) contrast (3.5-mm isotropic voxels, TE 30 ms, TR 2 s, flip angle 90°) were acquired from all participants. Each resting-state scan was 5-min long (150 vol). Subjects were instructed to rest quietly with eyes open.
9
Multimodal Neuroimaging Protocol for Brain Analysis
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Magnetic resonance imaging data were collected on a Siemens Magnetom Trio 3 T MRI system (Siemens Medical Solutions, Erlangen, Germany), using the Siemens 32-channel receive-only head coil at the Functional Neuroimaging Unit of the Research Center of the Institut universitaire de gériatrie de Montréal. A structural high-resolution T1-weighted 3D-Multi-Echo MPRAGE sequence (TR: 2530 ms; TE: 1.64 ms; flip angle: 7°; FoV: 256 mm; voxel size: 1.0 × 1.0 × 1.0 mm; 176 continuous slices) was acquired for volumetric analyses. A FLAIR weighted sequence (Fluid Attenuated Inversion Recovery; TR: 9000 ms; TE: 90 ms; flip angle: 150°; FoV: 240 mm; voxel size: 0.9 × 0.9 × 4.0 mm with 4.0 mm distance gap factor; 44 slices) was also obtained for WML confirmatory analysis.
10
High-resolution Structural and FLAIR MRI Acquisition
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Magnetic resonance imaging data were collected on a Siemens Magnetom Trio 3T MRI system (Siemens Medical Solutions, Erlangen, Germany), using the Siemens 32-channel receive-only head coil at the Functional Neuroimaging Unit of the Research Center of the Institut universitaire de gériatrie de Montréal. A structural high-resolution T1-weighted 3D-Multi-Echo MPRAGE sequence (TR: 2530 ms; TE: 1.64 ms; ip angle: 7°; FoV: 256 mm; voxel size: 1.0 x 1.0 x 1.0 mm; 176 continuous slices) was acquired for volumetric analyses. A Flair weighted sequence (Fluid Attenuated Inversion Recovery; TR: 9000 ms; TE: 90 ms; ip angle: 150°; FoV: 240 mm; voxel size: 0.9 x 0.9 x 4.0 mm with 4.0 mm distance gap factor; 44 slices) was also obtained for WML con rmatory analysis.
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