siRNAs targeting human 53BP1 (sc-37455) and BRG1 (sc-29827) and TOP2A (sc-36695) were obtained from Santa Cruz Biotechnology. siRNA pools targeting MDC1 (L-003506-00) and PICH (L-031581-01) were obtained from Dharmacon. Oligonucleotide mix targeting the 3′ UTR of endogenous human TOPBP1 (Dharmacon) contained the following oligos: 5′-GUAAAUAUCUGAAGCUGUAUU-3′, 5′-GCACAAGGUUUAAUGAGGAUU-3′, 5′-GCUGUAGCUUAGUGGAAAUUU-3′. Oligonucleotide targeting the 3′ UTR of BLM (Dharmacon) was as follows: 5′-GCUAGGAGUCUGCGUGCGAUU-3′. siRNA targeting TOP2B was 5′-UCGGGCUAGGAAAGAAGUAAA-3′50 (link):. Santa Cruz Biotechnology non-targeting siRNA control (sc-37007) or Dharmacon non-targeting smart pool siRNA (D-00180-10-20) were used as control siRNAs where appropriate. Oligonucleotides were transfected into human cells using Oligofectamine reagent (Life Technologies), according to the manufacturer’s instructions.
Oligonucleotide mix
Manufactured by Horizon Discovery
Oligonucleotide mix is a laboratory reagent composed of synthetic DNA or RNA fragments. It is designed for use in various molecular biology applications, such as gene expression analysis, DNA sequencing, and PCR amplification. The mix contains a combination of oligonucleotides, which are short sequences of nucleic acids that serve as essential components in these scientific procedures.
Automatically generated - may contain errors
Lab products found in correlation
Oligofectamine reagent, by Thermo Fisher Scientific (2 mentions)
Sirna pool, by Horizon Discovery (2 mentions)
Sc 37455, by Santa Cruz Biotechnology (2 mentions)
Sc 29827, by Santa Cruz Biotechnology (2 mentions)
L 003506 00, by Horizon Discovery (2 mentions)
Oligonucleotide targeting, by Horizon Discovery (2 mentions)
Non targeting sirna control sc 37007, by Horizon Discovery (2 mentions)
Non targeting smart pool sirna, by Horizon Discovery (2 mentions)
2 protocols using oligonucleotide mix
1
Targeting DNA Repair Proteins with siRNA
2
Targeting DNA Repair Proteins with siRNA
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