The specimen was fixed in 10 % formalin for 24 h after marker insertion. Block-face images of the whole specimen were obtained before and after fixation using an optical/digital camera (Nikon Coolpix 995, Nikon, Japan). The block-face images served as visual reference for the registration process. The specimen was then scanned ex vivo on the CT component of a SPECT-CT scanner (Philips Precedence 16, Eindhoven, Netherlands) with careful positioning such that the orientation differences between in vivo and ex vivo CT scans of the larynx were minimised.
Precedence 16
Manufactured by Philips
Sourced in Netherlands
The Precedence 16 is a compact and versatile laboratory equipment designed for general purpose applications. It features 16 individual temperature-controlled sample positions and provides precise temperature control and monitoring capabilities. The Precedence 16 is a reliable and efficient tool for researchers and scientists working in various laboratory settings.
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Lab products found in correlation
5 protocols using precedence 16
1
Ex Vivo Larynx Imaging Protocol
2
In Vivo Imaging of Aortic Atherosclerosis
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Three hours after injection of 99mTc-duramycin (74–111 MBq), rabbits were sedated and anesthetized with ketamine (20 mg/kg) and xylazine (5 mg/kg) and then placed in a supine position in a clinical dual-head SPECT/CT scanner (Precedence16, Philips Healthcare, Cleveland, OH, USA). SPECT acquisition covering from the aortic arch to the bifurcation was performed with 32 projections (10 s per projection) on the 140 keV photopeak of 99mTc with 15% windows, using a low-energy higher solution collimator. SPECT images were reconstructed into a matrix size of 128 × 128 pixels and a pixel size of 1.46 × 1.46 mm using the Astonish algorithm. A CT scan (100 mA, 130 kV, 1 mm-slice thickness) was acquired for subsequent SPECT/CT fusion. After in vivo imaging, animals were sacrificed with an overdose of pentobarbital, blood was drawn via cardiac puncture, and the aorta from the aortic arch to the iliac artery bifurcation was harvested and segmented at 1-cm intervals. Each segment was weighed and radioactivity concentration (decay corrected) was measured in an automatic gamma well counter (Zhongjia-1200 γ-counter, Zhongjia Guangdian Co. China). The results were expressed as the percentage of injected dose per gram of tissue (%ID/g). The harvested aortas were further processed for histological studies.
3
Radioactive Tracer-Based Molecular Imaging
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All chemicals and solvents were purchased commercially and used without further purification. 99mTcO4− was purchased from HTA Co. Ltd. (Changsha, China). HSA, ICG, and FITC were purchased from MERYER (Shanghai, China). Ultrafiltration tubes were purchased from Merck (New Jersey, USA). Radioanalysis was performed using a radiothin-layer chromatography scanner (Eckert@Ziegler, USA). Radioactivity was detected using a WIZARD 2480 γ-counter (PerkinElmer). SPECT imaging was performed using a clinical SPECT/CT system (Precedence 16; Philips, The Netherlands). CT and SPECT projections were reconstructed and analyzed using an EBW workstation (Philips, The Netherlands).
4
Dynamic Lymphoscintigraphy of Radiolabeled NPs
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Sprague Dawley rats were anesthetized with an oxygen flow of 1 L/min and 2% (v/v) isoflurane and fixed to a paperboard. After injection of 99mTc-ICG-HSA NPs (11.1 MBq/50 μL) into the left footpad, dynamic lymphoscintigraphy was performed immediately for up to 1 h using a SPECT/CT scanner (Precedence 16, Philips, Netherlands). Static planar scintigraphy was performed after 1 min of scanning at 2, 4, 24, and 48-hr post-injection, respectively. SPECT acquisition was performed with 60 steps in step-and-shoot mode, 20s per step, and a matrix size of 128 × 128. Regions of interest (ROI) were drawn on the acquired images to obtain time-radioactivity curves. Quantitative data were expressed as gamma counts. CT and SPECT projections were reconstructed and analyzed using an EBW workstation (Philips, The Netherlands).
5
Optimized Gated SPECT/CT Imaging Protocol
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A SPECT/CT system (Precedence 16; Philips) was used. The conditions for gated SPECT data acquisition were data acquisition time at 30 s/step (360°, 64 steps), R-R interval at 16, use of a vertex general-purpose collimator, and matrix sizes of 128 • 128 (pixel size, 3.19 mm) and 64 • 64 (6.39 mm) (concurrent imaging: different acquisition data can be stored at 1 imaging). Gated myocardial perfusion image reconstruction was performed using filtered backprojection (cutoff of 0.19 cycles/cm and order of 9 for a matrix size of 128 • 128) and a Butterworth filter for the preprocessing filter (cutoff of 0.37 cycles/cm and order of 9 for a matrix size of 64 • 64). A ramp filter was used as the reconstruction filter, without attenuation correction and scatter correction.
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