Ab135626

Manufactured by Abcam

Sourced in United States, Norway

Ab135626 is a monoclonal antibody. It is designed for use in immunoassays, including enzyme-linked immunosorbent assay (ELISA) and Western blotting applications.

Automatically generated - may contain errors

Lab products found in correlation

Whatman, by Cytiva (4 mentions) Ab151769, by Abcam (4 mentions) Pvdf membrane, by Cytiva (3 mentions) Passive lysis buffer (plb), by Promega (3 mentions) P stat5, by Cell Signaling Technology (2 mentions) Hrp conjugated anti rabbit secondary antibody, by Agilent Technologies (2 mentions) Stat5, by Santa Cruz Biotechnology (2 mentions) Creb 2 c 20, by Santa Cruz Biotechnology (2 mentions) β tubulin 9f3, by Cell Signaling Technology (2 mentions) Supersignal west femto maximum sensitivity substrate, by Thermo Fisher Scientific (1 mentions) Amersham imager 600, by GE Healthcare (1 mentions) Polyvinylidene difluoride membrane, by Cytiva (1 mentions) Ab4074, by Abcam (1 mentions) Ab97051, by Abcam (1 mentions) Ab8245, by Abcam (1 mentions) Amersham imager, by Cytiva (1 mentions) Two step detection kit, by Zhongshan Golden Bridge Biotechnology (1 mentions) Sc1377, by Santa Cruz Biotechnology (1 mentions) Ab64693, by Abcam (1 mentions) Sc 365858, by Santa Cruz Biotechnology (1 mentions)

7 protocols using ab135626

Western Blot Analysis of Protein Targets

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Protein analysis was performed via Western blotting on brain and kidney homogenates separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred to a polyvinylidene difluoride membrane (Whatman) and blotted for CGL (ab8245; Abcam), CBS (ab135626; Abcam), glyceraldehyde 3-phosphate dehydrogenase (GAPDH; ab8245; Abcam), and αTubulin (ab4074; Abcam), followed by horseradish peroxidase (HRP)-conjugated secondary anti-rabbit antibody (ab97051; Abcam) or anti-mouse antibody (62-6520; Invitrogen). Proteins were visualized by using SuperSignal West Femto Maximum Sensitivity Substrate (No. 34096; Thermo Scientific) on an Amersham Imager 600 (General Electric), and size was determined by using the PageRuler Plus Prestained (No. 26619; Thermo Fisher).

Yang J., Link C., Henderson Y.O., Bithi N, & Hine C. (2022). Peripubertal Bisphenol A Exposure Imparts Detrimental Age-Related Changes in Body Composition, Cognition, and Hydrogen Sulfide Production Capacities. Antioxidants & Redox Signaling, 36(16-18), 1246-1267.

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Comprehensive Protein Expression Analysis

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Protein analysis was performed via Western blot on tissue and cell homogenates in passive lysis buffer (Promega), separated by SDS-PAGE, transferred to PVDF membrane (Whatman) and blotted for CGL (ab151769 Abcam), CBS (ab135626 Abcam), 3MST (HPA001240 Sigma), Stat5 (sc-835 Santa Cruz), p-Stat5 (#9359 Cell Signaling Technology), GNMT (Aviva), AHCY (Abcam ab56146), ATF4 (aka CREB-2 C-20, Santa Cruz Biotechnology sc-200), ATG5 (Novus, NB110–53818), ATG7 (Sigma, A2856), β-Tubulin 9F3 (#2128 Cell Signaling) or Actin (#4970 Cell Signaling) followed by HRP conjugated secondary anti-rabbit antibody (Dako).

Hine C., Kim H.J., Zhu Y., Harputlugil E., Longchamp A., Matos M.S., Ramadoss P., Bauerle K., Brace L., Asara J.M., Ozaki C.K., Cheng S.Y., Singha S., Ahn K.H., Kimmelman A., Fisher F.M., Pissios P., Withers D.J., Selman C., Wang R., Yen K., Longo V.D., Cohen P., Bartke A., Kopchick J.J., Miller R., Hollenberg A.N, & Mitchell J.R. (2017). Hypothalamic-Pituitary Axis Regulates Hydrogen Sulfide Production. Cell Metabolism, 25(6), 1320-1333.e5.

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Immunohistochemical Analysis of Maxillary Molar

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After sacrifice, the trimmed maxillae were fixed in 10% neutral buffered formalin for 24 h. After decalcified in ethylenediaminetetraacetic acid for 4 weeks, the tissues were then embedded by paraffin. Four-micrometer consecutive horizontal sections were obtained from the middle to apical third of the maxillary first molar, and sections from similar position of the roots were used for histological study. Immunohistochemistry was performed with a two-step detection kit (Zhongshan Golden Bridge Biotechnology, Beijing, China) as previously described [39 (link)]. The positive staining cells were counted in five different slides from each sample (N = 5–6). The final result came from the average of three tests.
The primary antibodies were anti-CBS (1:100, ab135626, Abcam), anti-TNF-α (1:100, ab1793, Abcam), anti-IFN-γ (1:50, sc1377, Santa Cruz), anti-IL-10 (1:200; sc-365858, Santa Cruz, Dallas, TX), and anti-CD206 (1:200, ab64693, Abcam).

He D., Liu F., Cui S., Jiang N., Yu H., Zhou Y., Liu Y, & Kou X. (2020). Mechanical load-induced H2S production by periodontal ligament stem cells activates M1 macrophages to promote bone remodeling and tooth movement via STAT1. Stem Cell Research & Therapy, 11, 112.

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Comprehensive Protein Analysis Protocol

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Protein analysis was performed via western blot on tissue and cell homogenates in passive lysis buffer (Promega), separated by SDS-PAGE, transferred to PVDF membrane (Whatman) and blotted for CGL (ab151769 Abcam), CBS (ab135626 Abcam), 3MST (HPA001240 Sigma), Stat5 (sc-835 Santa Cruz), p-Stat5 (#9359 Cell Signaling Technology), GNMT (Aviva), AHCY (Abcam ab56146), ATF4 (aka CREB-2 C-20, Santa Cruz Biotechnology sc-200), ATG5 (Novus, NB110-53818), ATG7 (Sigma, A2856), β-Tubulin 9F3 (#2128 Cell Signaling) or Actin (#4970 Cell Signaling) followed by HRP conjugated secondary anti-rabbit antibody (Dako).

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Western Blot Analysis of Neurotransmitter Transporters

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All chemicals were purchased from Sigma-Aldrich (St Louis, MO, USA) unless otherwise indicated. Methylarsonic acid (MMAV) disodium salt (99% pure) was obtained from Chem Service (West Chester, PA, USA). Sodium borohydride was obtained from EM Science (Gibbstown, NJ, USA). For Western blots, primary rabbit antibodies against xCT (ab37185), EAAT3 (ab124802), GLAST (ab416), GLT1 (ab41621), NR2B (ab65783), GluA2 (ab133477), Synaptophysin (SY38, ab8049), CBS (ab135626), and CSE (ab151769) were obtained from Abcam (Cambridge, MA, USA). Anti-LAT1 (sc-134994) and PSD95 (7E3, sc-32290) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit anti-NR2A (AB1555P) and anti-GluA1 (AB1504) antibodies were purchased from Millipore (Bedford, MA, USA). Primary antibody against SLC1A4 (8442 s) and secondary goat anti-rabbit antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Secondary anti-mouse IgG antibody was purchased from Invitrogen (Waltham, MA, USA).

González-Alfonso W.L., Pavel P., Karina H.M., Del Razo L.M., Sanchez-Peña L.C., Zepeda A, & Gonsebatt M.E. (2023). Chronic exposure to inorganic arsenic and fluoride induces redox imbalance, inhibits the transsulfuration pathway, and alters glutamate receptor expression in the brain, resulting in memory impairment in adult male mouse offspring. Archives of Toxicology, 97(9), 2371-2383.

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Quantifying Sulfur Metabolism Proteins

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Total RNA was isolated from tissues and cells using miRNeasy Mini Kit (Qiagen) and cDNA synthesized by random hexamer priming with the Verso cDNA kit (Thermo). qRT-PCR was performed with SYBR green dye (Lonza) and TaqPro DNA polymerase (Denville). Fold changes were calculated by the ΔΔC_t method using Hprt and/or Rpl13 as standards and normalized to the experimental WT AL control. Protein expression was analyzed in tissues homogenized in passive lysis buffer (Promega), separated by SDS-PAGE, transferred to PVDF membrane (Whatman) and blotted for CGL (ab151769 Abcam), CBS (ab135626 Abcam) or Actin (13E5 Cell Signaling) followed by HPRT conjugated secondary anti-rabbit antibody (Dako).

Hine C., Harputlugil E., Zhang Y., Ruckenstuhl C., Lee B.C., Brace L., Longchamp A., Trevino-Villarreal J.H., Mejia P., Ozaki C.K., Wang R., Gladyshev V.N., Madeo F., Mair W.B, & Mitchell J.R. (2014). Endogenous Hydrogen Sulfide Production Is Essential for Dietary Restriction Benefits. Cell, 160(0), 132-144.

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Immunoprecipitation of Cystathionine β-Synthase

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Appropriate antibody (anti-human CBS, ab135626, Abcam) was incubated with 60 µL washed magnetic beads (Dynabeads M-280), coated with M-280 sheep anti-rabbit IgG (Invitrogen Dynal AS, Oslo, Norway) for overnight at 4°C on a rotator (VWR International, Radnor, PA, USA). As negative controls, the coated beads were incubated with either mouse IgG1_ (MOPC21, Sigma, USA), or with rabbit -globulin (Jackson ImmunoResearch, West Grove, PA, USA). The beads with attached antibody were washed (twice, 200 µL) with phosphate-buffered saline (PBS). Proteins were immunoprecipitated from 0.5 mg of detergentextracted total protein by incubation for 4 h at 4°C with antibody-bound beads. Bead complexes were washed four times with PTA solution (145 mmol/L NaCl, 10 mmol/L NaH 2 PO 4 , 10 mmol/L sodium azide, and 0.5% Tween 20, pH 7.0). Immunoprecipitated proteins were then extracted with 60 µL of 2x Laemmli sample buffer (Bio-Rad, Hercules, CA, USA) and boiled for 5 min.

Lišková V., Chovancova B., Babula P., Režuchová I., Pavlov K.P., Matúšková M., & Križanová O. (2020). Cystathionine-β-synthase affects organization of cytoskeleton and modulates carcinogenesis in colorectal carcinoma cells.

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