Adult C. elegans carrying eggs were dissected in a drop of egg buffer on polylysine-coated coverslips. The embryos were gently flattened by mounting with 22.8 mm beads (Whitehouse scientific, Chester, UK) as spacers. Images were acquired using a Nikon Eclipse Ti-2 microscope equipped with a 100X, 1.49 NA objective; a Photometrics Prime BSI camera; an OptoSpin filter wheel (CAIRN Research, Kent, England), and an vt-iSIM super-resolution confocal scan head (VisiTech international, Sunderland, UK). Confocal images were magnified by a 1.5× tube lens before being collected by camera chip. During image acquisition, the focal plane was centered at the cortex, and along with 2 slices above and below, 0.25 um per slice step, 5 total slices were collected. GFP was excited using a 488 nm laser and mScarlet was excited using a 561 nm laser.
Prime bsi camera
Manufactured by Cairn Research
Sourced in United Kingdom
The Prime BSI camera is a high-performance scientific imaging device designed for a variety of research and experimental applications. It features a backside-illuminated (BSI) sensor that provides enhanced sensitivity and low-noise performance. The camera's core function is to capture and record digital images or video, with the capability to operate in a range of wavelengths and lighting conditions.
Automatically generated - may contain errors
3 protocols using prime bsi camera
1
Live C. elegans Embryo Imaging
2
Live C. elegans Embryo Imaging
3
Imaging C.elegans embryos with TIRF-SIM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Adult C.elegans carrying eggs were dissected in a drop of egg buffer on polylysine-coated coverslips. The embryos were gently flattened by mounting with 22.8 mm beads (Whitehouse scientific, Chester, UK) as spacers. Images were acquired using a Nikon Eclipse Ti-2 microscope equipped with a 100X, 1.49 NA objective; a Photometrics Prime BSI camera; an OptoSpin filter wheel (CAIRN Research, Kent, England) , and an vt-iSIM super-resolution confocal scan head (VisiTech international, Sunderland, UK). Confocal images were magnified by a 1.5X tube lens before being collected by camera chip. During image acquisition, the focal plane was centered at the cortex, and along with 2 slices above and below, 0.25 um per slice step, 5 total slices were collected. GFP was excited using a 488 nm laser and mScarlet was excited using a 561 nm laser.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!