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Hitrap q strong anion exchange column

Manufactured by Bio-Rad
Sourced in United States

The HiTrap Q strong anion-exchange column is a pre-packed chromatography column designed for the purification and separation of biomolecules. It utilizes a strong anion-exchange resin to capture and selectively elute target molecules based on their charge properties. The column is suitable for various applications in the field of protein purification, nucleic acid separation, and other biomolecular analyses.

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2 protocols using hitrap q strong anion exchange column

1

Recombinant Protein Purification Protocol

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Malonyl-CoA, acetyl-CoA, acyl-CoAs, fatty acids, NADH, NADPH, and antibiotics were purchased from Sigma-Aldrich. Takara Biotechnology Co. provided molecular biology reagents. Novagen provided the pET vectors. Ni-agarose columns were obtained from Invitrogen. GE healthcare provided the HiTrap Q strong anion-exchange column and Bio-Rad provided the Quick Start Bradford dye reagent. All other reagents were of the highest available quality. Takara Biotechnology Co. synthesized the oligonucleotide primers.
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2

Biochemical Assay Reagents Procurement

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Malonyl‐CoA, acetyl‐CoA, acetoacetyl‐CoA, fatty acids, NADH, NADPH and antibiotics were purchased from Sigma (USA). Takara Biotechnology Co. (Japan) provided molecular biology reagents. Novagen (USA) provided pET vectors. Invitrogen (USA) provided Ni‐agarose columns. GE Healthcare (USA) provided the HiTrap Q strong anion‐exchange column and Bio‐Rad (USA) provided the Quick Start Bradford dye reagent. All other reagents were of the highest available quality. Oligonucleotide primers were synthesized by TaKaRa Biotechnology Co.
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