Male Crj: CD2F1 (BALB/c x DBA/2) mice were obtained from Charles River Laboratories Japan, Inc., Tokyo, Japan, and maintained in our animal facility. All mice were housed in metal cages (one mice per cage) and were fed a basal diet (Oriental CRF-1, Oriental Yeast Co., Ltd., Tokyo, Japan) and tap water ad libitum under controlled conditions of temperature (23 ± 1°C), humidity (55 ± 10%) and light (12-h light/12-h dark cycle). They were quarantined for one week and then randomized by body weight into each group.
Oriental crf 1
Manufactured by Oriental Yeast
Sourced in Japan
The Oriental CRF-1 is a laboratory equipment used for controlled-rate freezing of biological samples. It is designed to gradually lower the temperature of the samples at a pre-determined rate, ensuring the preservation of their structural and functional integrity during the freezing process.
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Lab products found in correlation
2 protocols using oriental crf 1
1
Mouse Housing and Husbandry Conditions
2
Murine Model of DSS-Induced Colitis
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Male Crj: CD2F1 (BALB/c × DBA/2) mice were obtained from Charles River Japan, Inc., Tokyo. All mice were housed in metal cages (one mouse per cage) and were fed a basal diet (Oriental CRF-1, Oriental Yeast Co., Tokyo, Japan) and tap water ad libitum, under controlled conditions of temperature (23 ± 3 °C), humidity (55 ± 20%), and light (12-h light/12-h dark cycle). They were quarantined and acclimatized for 1 week. The animals were assigned by stratified randomization to the two groups according to their body weights; mice from both the DSS- and CMNC/DSS-groups and both the non-treatment and CMNC groups were assigned to the heavy and light groups, respectively, to minimize the mortality of light mice, given that they are likely to be more sensitive to DSS-induced colitis than heavy mice.
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