Discovery mv690

Fluciclovine preparation was completed as earlier reported under IND (Investigational New Drug Application) 72,437.^{15 (link)} Patients ingested oral contrast medium after at least 4 hours of fasting. An initial cT with a 3.75 mm slice thickness and 3.25 mm spacing was completed to correct attenuation (approximately 100 mA). Next a mean ± SD of 364.1 ± 37.7 MBq (9.84 ± 1.02 mCi) intravenous fluciclovine was administered. Five minutes after injection the patient underwent dual time point PET from pelvis to diaphragm at 2.5 minutes per bed position for 4 table positions. Scanning was completed on a Discovery MV690 PET-CT scanner (GE Healthcare, Wauwatosa, Wisconsin). Images were reconstructed with an iterative technique using a VUE Point Fx (GE Healthcare) with 3 iterations, 24 subsets and a filter cutoff of 6.4 mm. Images were transferred to a MIMVista work station (MIM Software, Cleveland, Ohio) for interpretation.

Fluciclovine was prepared as earlier reported [21 (link), 24 (link)]. Patients were scanned on a GE Discovery MV690 PET-CT scanner after at least 4 hours of fasting to normalize amino acid levels. An initial abdominopelvic CT scan was conducted with administration of oral contrast only at 80–120 mA and 120 kVp. Following this, 8.6 – 10.78 millicuries (318.2 – 398.86 megabecquerel) of fluciclovine was injected intravenously over 2 minutes, with a 3-minute blood pool clearance window after which 5–15.5 minute and 16–27.5 minute images extending from the pelvis to the diaphragm were acquired and data transferred to a MIMVista workstation (MIM Software; Cleveland, OH) for analysis.

Details regarding ¹⁸F-fluciclovine PET/CT acquisition have been previously published [21 (link)]. Briefly, ¹⁸F-fluciclovine was synthesized as previously described [22 (link)]. A minimum of 4-hour fasting was observed by all patients. 10.0 ± 2.0 mCi ¹⁸F-fluciclovine was injected intravenously as a bolus followed by PET/CT imaging from the level of the diaphragm to the pelvis at 2.5 minutes per bed position. Imaging was acquired at two-time points; 5–15.5 minute and 16–27.5 minutes post tracer injection. Imaging was acquired on a hybrid Discovery MV690 PET/CT scanner (GE Healthcare, IL, USA). Image reconstruction was done using iterative algorithm, 3 iterations and 24 subsets, with a filter applied at 6.4mm.