Qtrap 6500

Manufactured by Thermo Fisher Scientific

Sourced in Germany

The QTRAP 6500 is a hybrid triple quadrupole/linear ion trap mass spectrometer designed for sensitive and robust quantitative and qualitative analysis. It features high-resolution quadrupole mass filters, a linear ion trap, and advanced ion detection capabilities.

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Lab products found in correlation

Lc 30ad, by Shimadzu (1 mentions) Acquity beh c18, by Waters Corporation (1 mentions) Ultimate 3000 system, by Thermo Fisher Scientific (1 mentions) Agilent 7000, by Agilent Technologies (1 mentions) Shim pack uflc cbm30a system, by Shimadzu (1 mentions) Scaa 104, by ANPEL (1 mentions) Cnwbond carbon gcb spe cartridge, by ANPEL (1 mentions) Shim pack uflc cbm30a, by Shimadzu (1 mentions)

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QTRAP 6500+ mass spectrometer (2 citations)

5 protocols using qtrap 6500

Comprehensive LC-MS Lipid Analysis

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For the reverse-phase liquid chromatography (LC), an UltiMate 3000-system from Thermo Fisher Scientific (Darmstadt, Germany) was employed. The chromatographic separation was performed according to Supplementary Methods on an Ascentis Express C18 main column (150 mm × 2.1 mm, 2.7 µm, Supelco) fitted with a guard cartridge (5.0 mm × 2.1 mm, 2.7 µm, Supelco). Samples were injected with a volume of 5 µL and analyzed in triplicates. The LC was coupled to a Q-Exactive HF (QEx HF) mass spectrometer (Thermo Scientific, Bremen, Germany) or a QTRAP 6500 (Applied Biosystems, Darmstadt, Germany) to use PRM or SRM acquisition mode (See Supplementary Methods).
To validate lipid mediator species identified with the QTRAP6500 (Applied Biosystems, Darmstadt, Germany) instrument, the QEx HF (Thermo Fisher Scientific, Bremen, Germany) was used to perform high resolution MS full scan (HR-FS) and data independent acquisition (DIA) analyses. DIA method preparation and data analysis were performed with Skyline (See Supplementary Methods).

Peng B., Kopczynski D., Pratt B.S., Ejsing C.S., Burla B., Hermansson M., Benke P.I., Tan S.H., Chan M.Y., Torta F., Schwudke D., Meckelmann S.W., Coman C., Schmitz O.J., MacLean B., Manke M.C., Borst O., Wenk M.R., Hoffmann N, & Ahrends R. (2020). LipidCreator workbench to probe the lipidomic landscape. Nature Communications, 11, 2057.

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Quantitative Analysis of Fluoroquinolones in Honey

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LC-MS/MS analysis was performed on an LC-30AD ultraperformance liquid chromatograph (Shimadzu, Japan) coupled with a QTRAP 6500 mass spectrometer (Applied Biosystems, Waltham, MA, USA). Chromatographic column (Waters ACQUITY BEH C18, 2.1 mm × 100 mm, 1.7 µm) was used for separating the FQs in honey samples. The mobile phases, consisting of A (methanol) and B (2 mmol/L ammonium formate containing 0.1% formic acid), were set at the flow rate of 0.3 mL/min. The elution gradient was as follows: 0–1.0 min (B: 95%), 1–3 min (B: 95–80%), 3–7.5 min (B: 80–5%), 7.5–10 min (B: 5%), 10–10.1 min (B: 5–95%), and 10.1–12.0 min (B: 95%). The injection volume of the sample extract was 5 µL. Source temperature was 500 °C and ion spray voltage was set at 4.5 kV; curtain gas, gas 1, and gas 2 pressures were set at 40 psi, 55 psi, and 50 psi, respectively. The MRM (multiple reaction monitoring) transition parameters are listed in the Supplementary Materials (Table S3).

He L., Shen L., Zhang J, & Li R. (2023). Comprehensive Investigation of Fluoroquinolone Residues in Apis mellifera and Apis cerana Honey and Potential Risks to Consumers: A Five-Year Study (2014–2018) in Zhejiang Province, China. Toxics, 11(9), 744.

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Quantitative Analysis of Environmental Pollutants

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Analysis of p,p’-DDE and dieldrin was conducted on an Agilent 7000 gas chromatography-tandem mass spectrometry (GC-MS/MS) (Santa Clara, CA) operating in the electron impact mode (70 eV). Analysis of TCS, TCC, and fipronil was accomplished on a liquid chromatography- tandem mass spectrometry (LC-MS/MS) using the QTRAP 6500 (Applied Biosystems, Framingham, MA) coupled to a Shimadzu Prominence UHPLC-30AD (Shimadzu Scientific Instruments, Inc., Columbia, MD), controlled by Analyst 1.6 software. Instrumental conditions are provided in Supporting Information. Multiple reaction monitoring (MRM) was used for quantitative analysis on both GC-MS/MS and LC-MS/MS. Transition ions of the target analytes and internal standards are summarized in Table S1.

Dang V.D., Kroll K.J., Supowit S.D., Halden R.U, & Denslow N.D. (2018). Activated Carbon as a Means of Limiting Bioaccumulation of Organochlorine Pesticides, Triclosan, Triclocarban, and Fipronil from Sediments Rich in Organic Matter. Chemosphere, 197, 627-633.

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Seed Metabolite Extraction and Analysis

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Seeds from each cultivar were collected from 5-6 plantlets as one sample. The collected seeds were washed three times with distilled water, naturally dried, and then stored at -80°C for further analysis.
The dried frozen seeds of one sample were mixed with zirconia beads then milled with a mixer (MM 400, Retsch) at 30 Hz for 1.5 min. For 100 mg of powder, 1 μl precooled methanol (70% v/v) was added into a 1.5 ml microcentrifuge tube and vortexed and stored at 4°C overnight for extraction. Extraction mixtures were centrifuged for 10 min at 4°C, then the supernatants were filtrated (SCAA-104, 0.22 μm pore size; ANPEL, Shanghai, China) and absorbed (CNWBOND Carbon-GCB SPE Cartridge, 250 mg, 3 ml; ANPEL, Shanghai, China) subsequently. Collected extraction samples were analyzed using an LC-ESI-MS/MS system (HPLC, Shim-pack UFLC Shimadzu CBM30A system; MS, Applied Biosystems QTRAP 6500).

Zhou Y., Wang Z., Li Y., Li Z., Liu H, & Zhou W. (2020). Metabolite Profiling of Sorghum Seeds of Different Colors from Different Sweet Sorghum Cultivars Using a Widely Targeted Metabolomics Approach. International Journal of Genomics, 2020, 6247429.

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Liquid Chromatography-Mass Spectrometry Metabolite Analysis

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The treated sera were analyzed using liquid chromatography on 2.6 μm, 2.1×100 mm Thermo C30 columns (Thermo Scientific, Waltham, MA, USA) using a ultra-performance liquid chromatography system (Shim-pack UFLC CBM30A; Shimadzu, Kyoto, Japan). The eluate was analyzed using mass chromatography (QTRAP 6500; Applied Biosystems, Foster, CA, USA).
Mobile phase A was 0.04% (v/v) acetic acid in water, and mobile phase B was 0.04% (v/v) acetic acid in acetonitrile; the flow rate was 0.35 mL.min⁻¹. Mobile phase gradient conditions were as follows: A/B (80: 20, v/v) 0 min, 70: 30 v/v 2.0 min, 40: 60 v/v 4 min, 15: 85 v/v 9 min, 10: 90 v/v 14 min, 5: 95 v/v 15.5 min, 5: 95 v/v 17.3 min, 80: 20 v/v 17.3 min, and 80: 20 v/v 20 min. The column and autosampler temperatures were maintained at 45°C and 4°C, respectively. The injection volume was 2 μL. Instrument tuning and mass calibration were performed using 10 and 100 μmol·L⁻¹ polypropylene glycol solutions. Scanning detection of ion pairs is based on optimized cluster potential and collision energy. The chemical structures and contents of its metabolites were analyzed using triple quadrupole scanning and multiple reaction monitoring techniques.

Zhou Y., Ding Y., Cui M., Zhang Y., Wang M., Zhou F., Su Y., Liang B, & Zhou F. (2024). Metabolomic Alterations in Methotrexate Treatment of Moderate-to-Severe Psoriasis. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 30, e943360-1-e943360-11.

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