miR-107 mimic (miR-107), mimic negative control (miR-NC), miR-107 inhibitor (anti-miR-107), inhibitor negative control (anti-miR-NC), pcDNA-ZNF217 overexpression vector (ZNF217) and pcDNA empty vector were obtained from Genepharma (Shanghai, China). PC12 cells were maintained in 6-well plates to reach 70% confluence and then transfected with 40 nM oligonucleotides or vectors using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). After transfection of 24 h, transfection efficacy was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) or Western blot.
Pcdna empty vector
Manufactured by GenePharma
Sourced in China
PcDNA empty vector is a plasmid that can be used as a cloning and expression vector. It provides a basic structure for inserting and expressing genes of interest in various cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions)
Mir nc, by GenePharma (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Mir in, by GenePharma (1 mentions)
Mir 381 3p in, by GenePharma (1 mentions)
Mir control, by GenePharma (1 mentions)
Si control, by GenePharma (1 mentions)
Anti mir 195, by GenePharma (1 mentions)
Pcdna hmga2, by GenePharma (1 mentions)
Si hmga2, by GenePharma (1 mentions)
Anti mir control, by GenePharma (1 mentions)
Pcdna hif 1α, by GenePharma (1 mentions)
Si linc01089, by GenePharma (1 mentions)
5 protocols using pcdna empty vector
1
Overexpression and Inhibition of miR-107 in PC12 Cells
2
MicroRNA-381-3p Regulates Neuroinflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The pcDNA empty vector (NC, 5 μg/ml), pcDNA-CCR2 (CCR2, 5 μg/ml), the miRNA control (miR-NC, 50 nM), miR-381-3p mimics (miR-381-3p, 50 nM), the miRNA inhibitor (miR-in, 100 nM), and the miR-381-3p inhibitor (miR-381-3p-in, 100 nM) were supplied by GenePharma Co., Ltd. (Shanghai, China). OGD-elicited BV2 microglia and HT22 hippocampal neurons were seeded into 24-well cell culture plates with a density of 3 × 105 cells/well. Transfection was conducted after they were cultivated in an environment of 37°C and 5% CO2 for 24 hours. The above-mentioned RNAs were transfected into OGD-induced BV2 microglia and HT22 neurons employing Lipofectamine® 3000 (Invitrogen; ThermoFisherScientific, Inc.). qRT-PCR was carried out to determine the efficiency of the transfection [20 (link)]. The cells were incubated under the conditions of 37°C and 5% CO2 for 24 hours in preparation for further analysis.
3
Regulation of HMGA2 by miR-195
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
EC109 and EC9706 cells were seeded in 6-well plates and transfected with miR-195 mimics, miR-control, anti-miR-195, anti-miR-control, pcDNA-HMGA2, pcDNA empty vector, si-HMGA2, or si-control (GenePharma, Shanghai, China) by Lipofectamine™ 2000 (Invitrogen, Carlsbad, CA, USA) following the manufacturer's protocol when the cells were grown to 80% confluency.
4
Targeting KLF5 and HIF-1α in Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
siRNAs specifically against KLF5 (si-KLF5#1 and si-KLF5#2), siRNA scrambled control (si-NC), pcDNA-KLF5, pcDNA-HIF-1α, and pcDNA empty vector (Vector) were purchased from GenePharma (Shanghai, China). Cells were plated in 6-well plates at a density of 2.5 × 105 cells/well and grown to 80% confluence. Subsequently, cell transfection with these oligonucleotides or plasmids was performed using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). At 48 h post-transfection, cells were harvested for further analysis.
5
LINC01089 Overexpression in HCC Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human HCC cell lines, Huh-7 and LM3, were purchased from the Cell Bank of Chinese Academy of Sciences (Shanghai, China). Cell lines were cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and kept in a humidified atmosphere at 37 °C with 5% CO2.
The pcDNA empty vector (Vector), pcDNA-LINC01089 overexpression vector (LINC01089), siRNA negative control (si-NC), and siRNAs against LINC01089 (si-LINC01089) were provided by GenePharma (Shanghai, China). Cell transfection was carried out according to the manufacturer’s instructions. After 6 h post-transfection, cells were cultured in fresh complete medium for subsequent experiments.
The pcDNA empty vector (Vector), pcDNA-LINC01089 overexpression vector (LINC01089), siRNA negative control (si-NC), and siRNAs against LINC01089 (si-LINC01089) were provided by GenePharma (Shanghai, China). Cell transfection was carried out according to the manufacturer’s instructions. After 6 h post-transfection, cells were cultured in fresh complete medium for subsequent experiments.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!