Generation of intracellular ROS was detected using the CellROX® Deep Red fluorescent probe (Invitrogen, Thermo Fisher Scientific Inc., Waltham, MA, USA). The cells were treated with IC90 of DT for 24 h and exposed to CellROX® Deep Red (5 μM, 30 min) at 26 °C in the dark. Cells were observed in a Leica TSC SPE confocal microscope equipped with inverted optics at λexc 633 and λem 519 nm.
Cellrox deep red fluorescent probe
Manufactured by Thermo Fisher Scientific
Sourced in Spain
The CellROX Deep Red fluorescent probe is a cell-permeant dye that can be used to measure oxidative stress in live cells. It exhibits strong fluorescence upon oxidation by reactive oxygen species (ROS) and reactive nitrogen species (RNS). The probe can be detected using far-red/near-infrared (Cy5) fluorescence imaging and flow cytometry techniques.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using cellrox deep red fluorescent probe
1
Intracellular ROS Detection via CellROX
2
Intracellular ROS Detection by CellROX
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The generation of intracellular ROS was detected using the CellROX Deep Red fluorescent probe (Invitrogen). The cells were treated with IC90 of STS for 24 h and exposed to CellROX Deep Red (5 μM, 30 min) at 26 °C in the dark. Cells were observed in a Leica TSC SPE- confocal microscope equipped with inverted optics at λexc = 633 and λem = 519 nm.
3
Intracellular ROS Detection Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The generation of intracellular ROS was detected using the CellROX® Deep Red fluorescent probe (Invitrogen). The cells were treated with IC90 of both 5 and 7 for 24 h and exposed to CellROX® Deep Red (5 μM, 30 min) at 26 °C in the dark. Cells were observed in a Leica TSC SPE-confocal microscope equipped with inverted optics at λexc = 633 and λem = 519 nm.
4
Intracellular ROS Measurement in Naegleria
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The generation of intracellular ROS was evaluated by using the CellROX Deep Red fluorescent probe (Invitrogen, Termo Fisher Scientific, Madrid, Spain). Naegleria fowleri trophozoites were treated with the IC90 of laurinterol for 24 h and exposed to CellROX Deep Red (5 μM, 30 min) at 37 °C in the dark. Cells were observed in an EVOS FL Cell Imaging System AMF4300 (Life Technologies, Madrid, Spain).
5
Measuring ROS in Treated Amoebae
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The generation of ROS in treated amoebae was determined with the CellROX Deep Red fluorescent probe (Invitrogen, Termo Fisher Scientifc, Madrid, Spain). The experiment was carried out after incubating 5*105 cells/ml with the IC90 of the tested compounds at 37 °C during 24 h. Afterwards, cells were exposed to CellROX Deep Red (final concentration 5 μM) and incubated for 30 min in dark. Finally, the presence or ROS was evaluated with EVOS FL Cell Imaging System AMF4300 (Life Technologies, Madrid, Spain) as they show high red fluorescence (Arberas-Jiménez et al., 2020 (link)).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!