Animal blood was collected during dissection. Samples were centrifuged for 10 minutes at 4000 × g to collect the serum. Red blood cells (RBC), hemoglobin (HGB), white blood cells (WBC), and platelets (PLT) were detected using a whole-blood analyzer. The alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine (Cr), and the electrolytes, such as sodium (Na) and potassium (K) were tested using an automated chemistry analyzer (FUJI DRICHEM 4000ie, Fujifilm Corporation, Tokyo, Japan).
Dri chem 4000ie
Manufactured by Fujifilm
Sourced in Japan
The DRI-CHEM 4000ie is a compact, bench-top clinical chemistry analyzer designed for rapid, accurate, and reliable analysis of a wide range of clinical chemistry parameters. It utilizes dry slide technology to perform a variety of tests, including tests for enzymes, electrolytes, and metabolites. The DRI-CHEM 4000ie is capable of processing multiple samples simultaneously and provides quick turnaround times for test results.
Automatically generated - may contain errors
7 protocols using dri chem 4000ie
1
Comprehensive Blood Analysis Protocol
2
Histopathological Analysis of Liver and Ileum
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For histopathologic analysis, formalin-fixed liver and ileum samples were paraffin-embedded, sectioned (5 μm thickness), and stained with hematoxylin and eosin. The sections were randomly numbered prior to reading and observed by an experienced pathologist. Images were acquired using a NanoZoomer 2.0-RS scanner (Hamamatsu Photonics KK, Hamamatsu City, Japan) equipped with scanner software. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining (Roche Diagnostics GmbH, Mannheim, Germany) was performed following the manufacturer's instruction and counterstained with 4′, 6-diamidino-2-phenylindole (DAPI). The alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities of serum samples were measured using a dry chemistry analyzer (FUJI DRI-CHEM 4000ie; Fujifilm Corporation, Tokyo, Japan) according to the manufacturer's instructions.
3
Comprehensive Hematology and Liver Function
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Blood was drawn for hematology analysis by a standard saphenous vein blood collection technique. A PCE-90Vet hematological autoanalyzer (HTI Inc., Powell, OH, USA) was used to determine hematological parameters such as hemoglobin (HGB), red blood cells (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), hematocrit (HTC), mean corpuscular hemoglobin concentration (MCHC), white blood cells (WBC), and platelets (PLT). Whole blood was centrifuged twice at 3000 rpm for 10 min to separate serum. Serum biochemical analysis was carried out with a DRI-CHEM 4000ie biochemical analyzer (Fuji Inc., Tokyo, Japan) to determine the levels of total bilirubin (TBIL), alkaline phosphatase (ALP), and alanine transaminase (ALT) in order to evaluate liver function.
4
Liver Enzyme and Biomarker Assessment
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Alanine aminotransferase aspartate (ALT) and aspartate aminotransferase (AST) were measured using FUJI DRI‐CHEM Slide GFP/ALT‐PIII and GOT/AST‐PIII, respectively, according to the manufacturer's instructions for DRI‐CHEM 4000ie (FUJIFILM). Serum hyaluronic acid (HA) and procollagen III‐N‐peptide (P‐III‐P) were measured by using radio‐immunoassay.
5
Histological and Biochemical Liver Evaluation
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Liver tissues were processed for paraffin embedding and sectioned into 4 μm sections. The sections were then routinely stained with haematoxylin and eosin (H&E staining). The serum levels of alanine aminotransferase aspartate (ALT) and aspartate aminotransferase (AST) were measured by using FUJI DRI-CHEM Slide GFP/ALT-PIII and GOT/AST-PIII, respectively, according to the manufacturer's instructions with DRI-CHEM 4000ie (FUJIFILM). Total bilirubin (TBiL) was measured using standard clinical chemistry techniques (Integra II; Roche, UK).
6
Hematoxylin-Eosin Staining of Liver Tissue
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Liver tissues were processed for paraffin embedding and were sectioned into 4 μm sections. The sections were then routinely stained with hematoxylin and eosin (H&E staining). The serum levels of alanine aminotransferase aspartate (ALT) and aspartate aminotransferase (AST) were measured by using FUJI DRI-CHEM Slide GFP/ALT-PIII and GOT/AST-PIII, respectively, according to the manufacturer's instructions with DRI-CHEM 4000ie (FUJIFILM).
7
Metabolic Biomarkers Profiling
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Glucose concentration was determined using the ACCU-CHEK (ROCHE, Switzerland) glucose analyzer. The levels of triglycerides (TG), total cholesterol (TC), high density lipoprotein (HDL), and low density lipoprotein (LDL) were determined using a DRI-CHEM 4000ie (Fujifilm, Japan). An enzyme-linked immunosorbent assay (ELISA) was used for measuring the levels of blood insulin (ALPCO, USA), OPN, and leptin (R&D Systems, USA).
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