Hipura plant dna isolation kit ctab method

The strain was isolated from mangrove tree leaves Kandelia candel (coast of Khanh Hoa province, Vietnam, South China Sea) on malt extract agar, and identified based on morphological and molecular features. For DNA extraction, the culture was grown on malt extract agar under 28 °C for 7 days. DNA extraction was performed with the HiPurA^TM Plant DNA Isolation kit (CTAB Method) (HiMedia Laboratories Pvt. Ltd., Mumbai, India) according to the manufacturer’s instructions. Fragments containing the ITS regions were amplified using ITS1 and ITS4 primers. The newly obtained sequences were checked visually and compared to available sequences in the GenBank database (https://www.ncbi.nlm.nih.gov/genbank, accessed on 16 December 2021). According to BLAST analysis of the ITS1–5.8S–ITS2 sequence, the strain LM.1.5 had 98.06% similarity with Aspergillus terreus DTO 403-C9 (sequence number in GenBank database—MT316343.1). The sequences were deposited in the GenBank nucleotide sequence database under MN788658.1.

The strain was isolated from an unidentified colonial ascidian (Shikotan Island, Pacific Ocean) on malt extract agar, and identified on the basis of morphological and molecular features. For DNA extraction, the culture was grown on malt extract agar under 25 °C for 7 days. DNA extraction was performed with the HiPurATM Plant DNA Isolation kit (CTAB Method) (HiMedia Laboratories Pvt. Ltd., Mumbai, India) according to the manufacturer’s instructions. Fragments containing the ITS (internal transcribed spacer) regions were amplified using ITS1 and ITS4 primers. The newly obtained sequences were checked visually and compared to available sequences in the GenBank database (www.mycobank.org). According to BLAST analysis of the ITS1–5.8S–ITS2 sequence, the strain KMM 4676 had 98% similarity with Aspergillus candidus. The sequences were deposited in the GenBank nucleotide sequence database under MG 241226. The strain is deposited in the Collection of Marine Microorganisms of G. B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS under the code KMM 4676.

The strain was isolated from brown algae Padina sp. (South China Sea, Vietnam) by the plating method using malt extract agar and identified on the basis of morphological and molecular features. For DNA extraction, the culture was grown on malt extract agar under 25 °C for 7 d. DNA extraction was performed by HiPurATM Plant DNA Isolation kit (CTAB Method) (HiMedia Laboratories Pvt. Ltd., Mumbai, India) according to the manufacturer′s instructions. Fragments containing the ITS regions were amplified using primers ITS1 and ITS4 [13 ]. Amplification of the partial calmodulin gene was performed using Cmd5 and Cmd6 primers [14 (link)]. The newly obtained sequences were checked visually and compared to available sequences of GenBank by using BLAST-n. According to BLAST analysis of the ITS1-5.8S-ITS2 and partial calmodulin datasets, the strain Penicillium sp. KMM 4672 is related to P. citrinum-group and displays the most similarity with P. steckii (99% and 97%, respectively). The sequences were deposited in GenBank nucleotide sequence database under KU 695807 and KU 695808. The strain was deposited in the Collection of Marine Microorganisms under the code KMM 4672.