Reagents used were 6-hydroxydopamine hydrobromide (6-OHDA, Sigma, USA), pargyline hydrochloride (MedChemExpress, USA), desipramine hydrochloride (MedChemExpress, USA), apomorphine (Absin Bioscience Inc, China), paraformaldehyde (PFA, Boster Biotechnology, China), RIPA lysis buffer (Beyotime Institute of Biotechnology, China), BCA Protein Assay Kit (Boster Biotechnology, China), ECL-enhanced chemoluminescence (Boster Biotechnology, China), Dopamine ELISA kit (Cloud-clone crop, China), TNF-α ELISA kit (Cloud-clone crop, China).
Semaglutide had been purchased from Bachem (Switzerland), and DA5-CH was synthetized by China peptides. The purity of each peptide was analyzed by reversed-phase HPLC and characterized using matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry, with a purity >97%. Peptides were reconstituted in ultrapure water (Milli-Q) to a concentration of 1 mg/ml, and aliquots were prepared and stored at −20°C.
Semaglutide had been purchased from Bachem (Switzerland), and DA5-CH was synthetized by China peptides. The purity of each peptide was analyzed by reversed-phase HPLC and characterized using matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry, with a purity >97%. Peptides were reconstituted in ultrapure water (Milli-Q) to a concentration of 1 mg/ml, and aliquots were prepared and stored at −20°C.