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Albumin from human serum

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Albumin from human serum is a laboratory product that serves as a source of albumin, the most abundant protein found in human blood plasma. Albumin plays a crucial role in maintaining the osmotic pressure of blood and transporting various substances, including hormones, vitamins, and drugs. This product is commonly used in various scientific and clinical applications that require a reliable source of human albumin.

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Lab products found in correlation

Sodium chloride (nacl), by Merck Group (3 mentions) Lc ms grade water, by Merck Group (2 mentions) Hexane, by Thermo Fisher Scientific (2 mentions) Phosphate buffered saline (pbs), by Merck Group (2 mentions) Ethyl acetate, by Thermo Fisher Scientific (2 mentions) Ammonium formate, by Merck Group (2 mentions) Lc ms grade methanol, by Merck Group (2 mentions) Albumin from bovine serum, by Merck Group (2 mentions) Sodium hydroxide (naoh), by Merck Group (2 mentions) Hydrogen peroxide, by Merck Group (2 mentions) Lc ms grade acetonitrile, by Merck Group (1 mentions) Formic acid for lc ms, by Merck Group (1 mentions) Lc ms grade 2 propanol, by Merck Group (1 mentions) Lysozyme, by AppliChem (1 mentions) Doxorubicin hydrochloride dox, by Merck Group (1 mentions) Congo red, by Merck Group (1 mentions) Magnesium nitrate hexahydrate, by Merck Group (1 mentions) Aluminum nitrate nonahydrate, by Merck Group (1 mentions) Methyl methanesulfonate, by Merck Group (1 mentions) Penicillin streptomycin, by Merck Group (1 mentions)

Spelling variants of this product

Human serum (542 citations) Human albumin (38 citations) Albumin from human serum (HSA; (5 citations) Albumins ( (2 citations) Fatty acid-free albumin from human serum (2 citations) Albumin from human serum (A9511) (2 citations)

19 protocols using albumin from human serum

1

Quantitative Ceramide Profiling by LC-MS

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Analytical grade chemical solvents, standards, and other materials were purchased from different companies. The powder forms of CerC16:0 [C16:0-ceramide (d18:1/16:0)], CerC18:0 [C18:0-ceramide (d18:1/18:0)], CerC22:0 [C22:0-ceramide (d18:1/22:0)], CerC24:0 [C24:0-ceramide (d18:1/24:0)], CerC24:1 [C24:1-ceramide (d18:1/24:1(15Z))] were purchased from Avanti Polar Lipids [Alabaster, AL]. The powder forms of Cer internal standards CerC16:0-d7 [C16:0-ceramide-d7 (d18:1-d7/16:0)], CerC18:0-d7 [C18:0-ceramide-d7 (d18:1-d7/18:0)], CerC24:0-d7 [C24:0-ceramide-d7 (d18:1-d7/24:0)], CerC24:1-d7 [C24:1-ceramide-d7 (d18:1-d7/24:1(15Z))] were also purchased from Avanti Polar Lipids [Alabaster, AL]. Albumin from human serum, phosphate-buffered saline, ammonium formate, and formic acid for LC-MS were obtained from Sigma-Aldrich [Burlington, Massachusetts, United States]. LC-MS-grade methanol, LC-MS-grade water, LC-MS-grade acetonitrile, and LC-MS-grade 2-propanol were purchased from Merck [United States]. Ethyl acetate and hexane were purchased from Fisher Scientific [Hampton, New Hampshire, United States]. The Acquity Ultra Performance Liquid Chromatography (UPLC) Ethylene Bridged Hybrid (BEH) C18 column (2.1 mm x 50 mm x 1.7 μm) was obtained from Waters [Milford, Massachusetts].
Shalaby Y.M., Al-Zohily B., Raj A., Yasin J., Al Hamad S., Antoniades C., Akawi N, & Aburawi E.H. (2024). Circulating ceramide levels and ratios in Emirati youth under 18 years: associations with cardiometabolic risk factors. Lipids in Health and Disease, 23, 93.
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2

Optimizing Protein Formulations via pH and Buffer Screens

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Albumin from human serum (10 mg/mL; Sigma, Vienna, Austria) and lysozyme (Applichem, Darmstadt, Germany) were dissolved into ddH2O. Antibody fragments were provided by Boehringer Ingelheim RCV GmbH and Co KG (Vienna, Austria). All samples are from the same protein batch, and several methods were applied under the same experimental conditions. The formulation screen was performed at different pH values and buffer stocks (0.5 M concentration) using the JBScreen Buffers (Jena Bioscience, Jena, Germany, Table S1), where SPG buffer (column 12) is produced by mixing succinic acid:sodium dihydrogen phosphate:glycine in the molar ratio 2:7:7.
Zhang F., Richter G., Bourgeois B., Spreitzer E., Moser A., Keilbach A., Kotnik P, & Madl T. (2021). A General Small-Angle X-ray Scattering-Based Screening Protocol for Studying Physical Stability of Protein Formulations. Pharmaceutics, 14(1), 69.
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3

Doxorubicin-Albumin Nanoparticle Synthesis

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Congo Red (CR, 96% purity, Aldrich Chemical Company, Inc., Milwaukee, WI 53233 USA), doxorubicin hydrochloride (Dox, 98% purity, SIGMA–ALDRICH, Co., 3050 Spruce Street, St. Louis, MO 63103, USA), albumin from bovine serum (BSA, 96% purity, SIGMA–ALDRICH, Co., 3050 Spruce Street, St. Louis, MO 63103 USA), albumin from human serum (HSA, 96% purity, SIGMA–ALDRICH, Co., 3050 Spruce Street, St. Louis, MO 63103 USA). All other reagents used were of analytical grade and were purchased from commercial sources.
Jagusiak A., Chłopaś K., Zemanek G., Kościk I., Skorek P, & Stopa B. (2022). Albumin Binds Doxorubicin via Self–Assembling Dyes as Specific Polymolecular Ligands. International Journal of Molecular Sciences, 23(9), 5033.
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4

Synthesis of Metal Nitrate Hydrates

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Magnesium nitrate hexahydrate (Mg(NO3)2·6H2O), aluminum nitrate nonahydrate (Al(NO3)3·9H2O), albumin from human serum were purchased from Sigma-Aldrich, Inc. (St. Louis, MO, USA). Sodium hydroxide (NaOH) and sodium bicarbonate (NaHCO3) were obtained from Daejung Chemicals & Metal Co., Ltd. (Siheung, Korea).
Jung S.Y., Kim B.K., Kim H.J, & Oh J.M. (2021). Development of Mesopore Structure of Mixed Metal Oxide through Albumin-Templated Coprecipitation and Reconstruction of Layered Double Hydroxide. Nanomaterials, 11(3), 620.
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5

Hematopoietic Cell Culture Protocol

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Horse serum was purchased from Biochrom AG (Berlin, Germany). The protein-assay dye reagent concentrate was from Bio-Rad (Munich, Germany) and the GelGreen nucleic acid gel stain was from Biotium (Hayward, CA, USA). Hematopoietic Growth Medium (HPGM) was from Lonza (Cologne, Germany). Recombinant human fms-related tyrosine kinase 3 ligand (Flt3), purified recombinant human stem cell factor (SCF) and recombinant human thrombopoietin (TPO) were from MACS Miltenyi Biotec (Gladbach, Germany). Mitomycin C was from Medac (Hamburg, Germany) and methanol was from Carl Roth (Karlsruhe, Germany). 1,4-Diazabicyclo[2.2.2]octane (DABCO), albumin from human serum, cytochalasin B, L-glutamine solution, methyl methanesulfonate, penicillin-streptomycin (10,000 units penicillin and 10 mg streptomycin per ml), RPMI 1640 medium (HEPES modification) and sodium pyruvate solution were purchased from Sigma-Aldrich (Munich, Germany). Doxorubicin hydrochloride, Mitomycin C and vinblastine sulfate were from Teva (Ulm, Germany).
Hintzsche H., Montag G, & Stopper H. (2018). Induction of micronuclei by four cytostatic compounds in human hematopoietic stem cells and human lymphoblastoid TK6 cells. Scientific Reports, 8, 3371.
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6

Antibody-Based Detection of Apoptosis Markers

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Anti-caveolin-1 antibody (N-20) was purchased from Santa Cruz
Biotechnology (Santa Cruz, CA). Anti- cleaved caspase-9, cleaved PARP, human
albumin, beta actin and GAPDH antibodies were purchased from Cell Signaling
Technology (Danvers, MA). Albumin from human serum was purchased from
Sigma-Aldrich (St. Louis, MO). Abraxane(R) (nab-paclitaxel) was
supplied as lyophilized powder by Celgene (Summit, NJ). For in
vitro and in vivo studies, nab-paclitaxel was
dissolved in normal saline (0.9% NaCl in distilled water). MIAPaCa-2,
BxPC3, AsPC1, HPAFII, FHs74 Int and Capan-2 cells were obtained from and
authenticated (via short tandem repeat profiling) by the American Type Culture
Collection (Manassas, VA), and grown according to ATCC recommendations. A549,
H23, H1299, H520, H792 cells were kindly provided by Wenrui Duan at the Ohio
State University. HBEC3KT cells were provided by David Carbone at Ohio State
University. Cells used for this study were cryopreserved after authentication by
short tandem repeat profiling. Cells were passaged for no longer than 3 months
and grown in a 37°C incubator with 5% CO2.
Chatterjee M., Ben-Josef E., Robb R., Vedaie M., Seum S., Thirumoorthy K., Palanichamy K., Harbrecht M., Chakravarti A, & Williams T.M. (2017). Caveolae-Mediated Endocytosis is Critical for Albumin Cellular Uptake and Response to Albumin-Bound Chemotherapy. Cancer research, 77(21), 5925-5937.
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7

Magnetic Bead-Based Protein Purification

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Carboxylated magnetic beads (MB, 1 μm diam., 20 mg/mL) were from Polysciences, Inc. Glutathione S-transferase (GST) from human placenta, albumin from human serum, and bovine serum albumin were from Sigma-Aldrich. Pierce trypsin protease, Pierce Glu-C protease, and Pierce chymotrypsin protease (TLCK treated) were from ThermoFisher Scientific. Sources of all chemicals and full experimental details are in the Supporting Information (SI) file.
Jiang D., Shen M., Ahiadu B, & Rusling J.F. (2020). Organ-Specific Screening for Protein Damage Using Magnetic Bead Bioreactors and LC–MS/MS. Analytical chemistry, 92(7), 5337-5345.
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8

Liquid Chromatography-Mass Spectrometry Assay for Vitamin D Metabolites

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25-hydroxyvitamin D3 (6, 19, 19-d3), 1-α-25-dihydroxyvitamin-D2 (1α,25(OH)2D2), 1-α-25 dihydroxyvitamin-D3 (1α,25(OH)2D3), 25-hydroxyvitamin-D2 (25OHD2), 3-epi-25- hydroxyvitamin-D2 (3-epi-25OHD2), 25-hydroxyvitamin-D3 (25OHD3), 3-epi-25- hydroxyvitamin-D3 (3-epi-25OHD3), vitamin-D2, vitamin-D3, 7- α-hydroxy-4-cholesten-3-one (7αC4), phosphate buffered saline, albumin from human serum, formic acid and ammonium formate for LC–MS were purchased from Sigma-Aldrich. LC–MS-grade methanol and LC–MS-grade water were purchased from Merck [United States]. Hexane and ethyl acetate were purchased from Fisher Scientific [Hampton, New Hampshire, United States].
Alzohily B., AlMenhali A., Gariballa S., Munawar N., Yasin J, & Shah I. (2024). Unraveling the complex interplay between obesity and vitamin D metabolism. Scientific Reports, 14, 7583.
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9

Synthesis and Characterization of Iron-Humate Complexes

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Iron(II) chloride tetrahydrate (99+%), iron(III) chloride hexahydrate (99+%) and ammonium hydroxide (25% solution in water), hydrochloric acid (37%), and sodium hydroxide (98%) were purchased in Acros Organics™, Geel, Belgium. Sodium chloride (99+%), albumin from human serum (99+%) and glucose (99.5+%) were purchased from SigmaAldrich, St. Louis, MO, USA. All chemicals were used without further purification.
Commercially available potassium humate from leonardite (a highly oxidized variety of lignites) (Powhumus®, Humintech GmbH, Grevenbroich, Germany) was used. Its basic properties, such as elemental and functional group analyses, 13C NMR spectroscopy, and size exclusion chromatography have been reported earlier elsewhere [26 (link)].
Kostyukhin E.M., Nissenbaum V.D., Abkhalimov E.V., Kustov A.L., Ershov B.G, & Kustov L.M. (2020). Microwave-Assisted Synthesis of Water-Dispersible Humate-Coated Magnetite Nanoparticles: Relation of Coating Process Parameters to the Properties of Nanoparticles. Nanomaterials, 10(8), 1558.
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10

Antioxidant and Anti-inflammatory Assays

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DPPH·, indomethacin, N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD), arachidonic acid from porcine liver, cyclooxygenase 1 from sheep, cyclooxygenase 2 human recombinant, trichloroacetic acid (TCA), 2-thiobarbituric acid (TBA), 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC), chlorogenic acid, (−)-epicatechin, deuterium oxide (D2O), 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH), and albumin from human serum (lyophilized powder, essentially fatty acid free) were purchased from Sigma–Aldrich (Poznań, Poland). Egg yolk phosphatidylcholine (PC) was obtained from Lipid Products, UK. The probes DPH, DPH-PA, TMA-DPH, and Laurdan were purchased from Molecular Probes (Eugene, Oregon). Tris (hydroxymethyl) aminomethane (Tris:HCl) were obtained from “Chempur” Piekary Śląskie. Bacteria cultures (L. casei PCM 2639 and L. plantarum PCM 2675) were from the Polish Collection of Microorganisms (PCM, Institute of Immunology and Experimental Therapy, Polish Academy of Science in Wrocław).
Strugała P., Cyboran-Mikołajczyk S., Dudra A., Mizgier P., Kucharska A.Z., Olejniczak T, & Gabrielska J. (2016). Biological Activity of Japanese Quince Extract and Its Interactions with Lipids, Erythrocyte Membrane, and Human Albumin. The Journal of Membrane Biology, 249, 393-410.
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