Haucl4 3h2o

All reagents used for synthesis were of analytical grade. Re-distilled water was used throughout. A stock solution of Au (1000 mg L⁻¹) was prepared by dissolving HAuCl₄ × 3H₂O (Merck, Germany) in re-distilled water. Working solutions of Au at concentrations of 50, 100, and 200 mg L⁻¹ were prepared from the stock solution by appropriate dilutions with water. The conductivity of all solutions of Au was adjusted with a concentrated 37 % (m/m) HCl solution (Avantor Performance Materials, Poland). The final concentration of HCl in these solutions was 0.10 mol L⁻¹. To prevent the agglomeration and the sedimentation of Au NPs, GEL (average molecular weight 80,000 g mol⁻¹, 99.9 % purity, Rousselot, USA), PVP (average molecular weight 40,000 g mol⁻¹, 99.9 % purity, Sigma-Aldrich, Poland), and PVA (average molecular weight 89,000–98,000 g mol⁻¹, 99.9 % purity, Sigma-Aldrich, Poland) were added to solutions. Final concentrations of mentioned stabilizers in these solutions were 0.5 % (m/v).

For all of the experiment steps, distilled water was employed. The following chemicals were utilized in this investigation: trisodium citrate (Trisodium 2-hydroxypropane-1,2,3-tricarboxylate), poly (vinyl alcohol), HAuCl₄.3H₂O,
p-aminohippuric acid, Ga(NO₃)₃.9H₂O, glycine were obtained from Merck (Darmstadt, Germany). Various concentrations (34.9–418.3 μg/L) of Ga(III) were carefully prepared by serial dilutions of Ga(NO₃)₃.9H₂O stock solution to reach the lowest concentration. The
p-aminohippuric acid (or N-(4-Amino benzoyl) glycine) solution (1.0 × 10^–4 M) was meticulously prepared by dissolving the convenient quantity of PAH in 8.0 × 10^–3 M NaOH. Glycine-HCl buffer (Gly-HCl) (0.1 M, pH 2.2–3.6) was exactly prepared by mixing 0.2 M Gly with 0.2 M HCl diluted to make up a total volume of 100 mL. All the metal salts used in the experiments were of analytical grade and dissolved in 0.01M HCl.

4‐mercaptobenzoic acid (MBA), 7‐mercapto‐4‐methylcoumarin (MMC), and 5,5′‐dithiobis‐(2‐nitrobenzoic acid) (DTNB) were provided by Merck. 2,3,5,6‐tetrafluoro‐4‐mercaptobenzoic acid (TFMBA) was supplied by TCI (Japan). AuNPs were synthesized with HAuCl₄·3H₂O and sodium citrate tribasic dihydrate, both were ordered from Merck. IgG isotype control (monoclonal mouse IgG₁, MAB002), anti‐EGFR monoclonal antibody (cetuximab biosimilar, recombinant monoclonal human IgG₁ clone, MAB9577), anti‐EpCAM (monoclonal mouse IgG_2B Clone, MAB9601), anti‐HER2 (monoclonal mouse IgG_2B clone, MAB1129), and anti‐HER3 (monoclonal mouse IgG₁ clone, MAB3481) were provided by R&D systems (Australia). The same antibodies were used for both SERS and flow cytometry. 3,3′‐dithiobis(sulfosuccinimidyl propionate) (DTSSP) were provided by Merck for conjugation of antibodies with AuNPs. The secondary antibody Alexa Fluor 488 goat anti‐mouse IgG antibody was ordered from Life Technologies (A‐11001, Australia), human IgG APC‐conjugated antibody (F0135) was supplied by R&D systems. Cell line SW480 and SW48 were supplied by ATCC.

PPG (Mn = 4000 g mol⁻¹) and NaBH₄ were purchased from Sigma-Aldrich, Germany, HAuCl₄ · 3H₂O from Merck Chemicals, Germany, and HPLC grade methanol from Tedia, USA. CPH standard was taken from local pharmaceutical company and commercial samples of the drug were purchased from a local pharmacy.
The glassware was washed with 10% nitric acid to minimize the contamination risk, and afterwards, rinsed with distilled water followed by drying in the oven. The pH meter from Laqua Horbia (pH 1300) was used having glass working and Ag/AgCl reference electrode. A double-beam spectrophotometer (CECIL 7400) was used to record UV-visible spectra in the region of 300 to 800 nm by using a quartz cuvette having a path length of 1 cm. FT-IR (Bruker Vector 22) having deuterated triglycine sulfate detector was used to record the spectra in the region of 400–4000 cm⁻¹ using KBr disc and 10 scans with a spectral resolution of 0.1 cm⁻¹ were recorded.
To determine the particle size and zeta potential, nano-ZSP (Malvern Instruments) (zeta sizer) was used. AFM (Agilent 5500) in tapping mode was used to record topographical images of PPG-AuNPs. For the sample preparation, one drop of the sample was placed on a silicon wafer that is air-dried for 24 h. The triangular nitride silicon cantilever (Veeco, model MLCT-AUHW) was used for the analysis of the sample under a spring constant value of 0.1 N m⁻¹.

Al(NO₃)₃ • 9H₂O (Merck, Warsaw, Poland), ACS reagent, ≥98% (Merck, Poland), 1,4-naphtalene dicarboxylic acid (1,4-NDC), 98% (AmBeed Inc., Arlington, IL, USA), HAuCl₄ • 3H₂O), ACS reagent, ≥49.0% Au basis (Merck, Poland), 1-octadecanethiol ≥98% (Merck, Poland), tetraoctylammonium bromide [CH₃ (CH₂)₇]₄NBr TOAB, 98% (Merck, Poland), tetrabuthylammonium bromide ([CH₃ (CH₂)₃]₄NBr) TBAB, 98% (Merck, Poland), NaBH₄, powder ≥ 98% (Sigma Aldrich, Poznań, Poland), glycidol, (Gly) 96% (Thermo Scientific, Bionovo, Legnica, Poland), Epichlorohydrine (EPI), 99% (Thermo Scientific, Bionovo, Poland), Styren oxide (St-O) ≥ 97% (Thermo Scientific, Bionovo, Poland), (+/−) Propylene oxide (Pr-O), ≥99% (Thermo Scientific, Bionovo, Poland), dimethylformamide DMF (>95%) (Chemat, Gdansk, Poland), dichlormethane (DCM) > 95%, (Chemat, Poland), ethyl acetate > 95%, (Chemat, Poland), toluene (>95%) (Chemat, Poland), ethanol anhydrous (≥99%) (Chemat, Poland), 4H-1,2,4-triazol-4-amine (4-Atrz), 95% (AmBeed, Inc., Arlington, USA), 5-amino-1H-tetrazole (5-Atz), 95% (Angene Chemical, Hyderabad, India), anizole anhydrous, 99.7%, (Merck, Poland), propylene carbonate, anhydrous 99.7%, (Merck, Poland), 4-(Hydroxymethyl)-1,3-dioxolan-2-one, ≥98%, (Merck, Poland), methanol anhydrous, 99% (Merck, Poland).

Myricetin was purchased from Sigma-Aldrich Co. and used as received. Stock solution of Myricetin (1.0×10⁻³ mol L⁻¹) was prepared in ethanol as solvent and diluted with 0.1 M phosphate medium (pH 3.5) before use. Multi-wall carbon nanotubes (diameter: 10–20 nm, length: 1–2 µm, purity >95%) were obtained from Sigma-Aldrich Co. Gold nanoparticles with average diameters of ∼13 and ∼40 nm were prepared by the reduction of HAuCl₄.3H₂O (Merck) with sodium citrate. Other chemicals were analytical grade and used without further purification in double-distilled water.

All
chemicals were used
directly as provided commercially without additional purification.
Milli Q water was obtained using an IQ 7000 purifying system. P25
TiO₂, HAuCl₄·3H₂O, PdCl₂, acetone (99.9%, CH₃COCH₃), sodium
hydroxide (reagent grade, 97%, powder, NaOH), 1-phenylethanol (97%),
hexanoic acid (99%), pentane (99%), lauric acid (97%), palmitic acid
(99%), stearic acid (98%), oleic acid (97%), undecane (99%), pentadecane
(99%), heptadecane (99%), p-xylene (99.5%, as internal
GC standard), different solvents including dodecane (99%), PhMe (99%),
THF (99%), DMF (99%) and dichloroethane (99%) were purchased from
Sigma-Aldrich. Jatropha oil was purchased from the Shenyu company
in Yunnan Province, China. Wasted cooking oil and wasted hot-pot oil
were collected from Sichuan Province, China.