Sodium chloride, methanol, ethanol, amino acid standards, acetonitrile, phenyl isothiocyanate, gallic acid, triethylamine, n-hexane, boron trifluoride-methanol, dichloromethane, formic acid, diethyl ether, petroleum ether, sodium hydroxide, potassium hydroxide, hydrochloric acid, and perchloric acid were purchased from ANPEL Laboratory Technologies (Shanghai) Inc. Vitamin B1/B2 and vitamin E were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. Folin-Ciocalteu reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), mixed standards of fatty acids, cyanidin 3-O-glucoside, anthranone, 1,2,3-trihydroxybenzene, benzoyl-dl -arginine p-nitroanilide hydrochloride, dimethyl sulfoxide, sulfosalicylic acid, trypsin, and phytic acid were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). The purity of chemicals used for High-Performance Liquid Chromatography (HPLC) and Gas Chromatography Mass Spectroscopy (GCMS) complied with the requirement of relevant procedures. All other chemicals were of analytical grade.
Vitamin e
Manufactured by Yuanye Bio-Technology
Sourced in China
Vitamin E is a fat-soluble compound that functions as an antioxidant. It helps protect cells from damage caused by free radicals.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Ethanol, by ANPEL (1 mentions)
Dichloromethane, by ANPEL (1 mentions)
Phytic acid, by Merck Group (1 mentions)
N hexane, by ANPEL (1 mentions)
Boron trifluoride methanol, by ANPEL (1 mentions)
Folin ciocalteu reagent, by Yuanye Bio-Technology (1 mentions)
1 2 3 trihydroxybenzene, by Merck Group (1 mentions)
Sulfosalicylic acid, by Merck Group (1 mentions)
Cyanidin 3 o glucoside, by Merck Group (1 mentions)
Trypsin, by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Methanol, by ANPEL (1 mentions)
Acetonitrile, by ANPEL (1 mentions)
Formic acid, by ANPEL (1 mentions)
Gallic acid, by ANPEL (1 mentions)
Methionine, by Yuanye Bio-Technology (1 mentions)
Phenol red, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Taurine, by Yuanye Bio-Technology (1 mentions)
6 protocols using vitamin e
1
Comprehensive Chemical Reagent Procurement
2
Antioxidant Compounds and Cell Viability Study
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Taurine, methionine, vitamin E, vitamin D, malic acid, citric acid, rutin, quercetin, ferulic acid, and chlorogenic acid (98% pure) were obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China), and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide (MTT), HEPES, 2′, 7′-dichlorofluorescin diacetate (DCFH- DA), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich, Inc. (St. Louis, USA). Dulbecco’s modified Eagle’s medium (DMEM) with glucose, phenol red, fetal bovine serum (FBS), phosphate-buffered saline (PBS), penicillin, and streptomycin were purchased from Gibco Life Technologies (Grand Island, NY, USA). A bicinchoninic acid assay kit, trypsin, and NP-40 buffer were obtained from Beyotime Biotechnology Inc. (Beijing, China). All other chemicals used were of analytical grade and commercially available in China.
3
Synthesis and Characterization of Tungsten-Titanium Oxide Nanocomposites
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All chemicals used in this study were analytical grade without any purification treatment. Sodium tungstate dihydrate (Na2WO4·2H2O) and tetrabutyl titanate (C16H36O4Ti) were purchased from Aladdin. Vitamin E was purchased from Yuanye Bio-Technology. Hydrochloric acid (HCl), sulfuric acid (H2SO4), acetone (CH3COCH3), methanol (CH3OH), isopropyl alcohol (C3H8O, IPA), and silver nitrate (AgNO3) were purchased from Beijing chemical plant. Terephthalic acid was purchased from InnoChem. Dibenzoyl dihydrazine, sodium sulfate (Na2SO4), sodium hydroxide (NaOH), ethylenediaminetetraacetic acid disodium salt (EDTA-2Na), N,N-dimethylformamide (DMF), and phosphoric acid (H3PO4) were purchased from Fuchen Chemical.
4
Formulation and Characterization of SPI-based Emulsion
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SPI (protein 90.13%, w/w) was supplied by Yousuo Chemical Technology Co., Ltd. (Shandong, China). Corn oil was obtained from a local market (Harbin, China). Carrageenan (CAS#9000-07-1, intensity 1200), gum arabic, and vitamin E were purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China). Distilled water was used, and all other chemicals used in the study were of analytical grade.
5
Goat Semen Collection and Analysis
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In this experiment, an artificial vagina was used to collect goat semen, and sperm motility analysis was conducted on the collected semen. Six goat semen with sperm motility higher than 80% were mixed, and the mixed semen was diluted eight times. The diluted semen was stored at a constant temperature of 4°C and used for detection and analysis.
The components of semen diluent were as follows: SS 0.16 to 0.8 mg or CC 0.04 to 0.80 mg, vitamin E 4 mL, penicillin sodium 15,000 IU, streptomycin sulfate15,000 IU, which were all bought from Shanghai yuanye Bio-Technology Co., Ltd (Shanghai, China). Fructose 1.26 g and citric acid 1.72 g, which were all bought from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). Trimethyl aminomethane 3.53 g, vitamin C 0.5 g, bovine serum albumin 0.5 g, which were all bought from Beijing Solarbio Science & Technology Co., Ltd (Beijing, China). Fresh egg yolk 20 mL, double-distilled water up to 100 mL. Diluent components are classified as analytical or guaranteed grade reagents.
The components of semen diluent were as follows: SS 0.16 to 0.8 mg or CC 0.04 to 0.80 mg, vitamin E 4 mL, penicillin sodium 15,000 IU, streptomycin sulfate15,000 IU, which were all bought from Shanghai yuanye Bio-Technology Co., Ltd (Shanghai, China). Fructose 1.26 g and citric acid 1.72 g, which were all bought from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). Trimethyl aminomethane 3.53 g, vitamin C 0.5 g, bovine serum albumin 0.5 g, which were all bought from Beijing Solarbio Science & Technology Co., Ltd (Beijing, China). Fresh egg yolk 20 mL, double-distilled water up to 100 mL. Diluent components are classified as analytical or guaranteed grade reagents.
6
Casein-Hyaluronic Acid-Vitamin E Formulation
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Casein powder (80 % protein) was obtained from Sigma-Aldrich. Hyaluronic acid (194 kDa) was obtained from Focusfreda Biological Co., ltd. (Shandong, China). Vitamin E was purchased from Shanghai Yuanye Bio-Technology Co., ltd. (Shanghai, China). Other chemicals were of analytical grade.
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